Skip to main content
SpringerLink
Log in

Skeletal Muscle Stem Cells

  • Protocol
  • First Online:
Cellular Cardiomyoplasty

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1036))

Abstract

Skeletal muscle satellite cells (myoblasts) are the primary stem cells of skeletal muscle which contribute to growth, maintenance, and repair of the muscles. Satellite cells are the first stem cells used for cellular cardiomyoplasty more than 20 years ago. The isolation, culture, labeling, and identification of satellite cells are described in detail here. The implantation and outcomes of cellular cardiomyoplasty using satellite cells have been summarized in the previous chapter (Chapter 1).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Mauro A (1978) Muscle regeneration. Raven, New York

    Google Scholar 

  2. Brack AS, Rando TA (2012) Tissue-specific stem cells: lessons from the skeletal muscle satellite cell. Cell Stem Cell 10:504–514

    Article  PubMed  CAS  Google Scholar 

  3. Carlson BM, Faulkner JA (1983) The generation of skeletal muscle fibers following injury: a review. Med Sci Sports Exerc 15:187–198

    Article  PubMed  CAS  Google Scholar 

  4. Snow MH (1978) An autoradiographic study of satellite cells differentiation into regenerating myotubes following transplantation of muscle in young rat. Cell Tissue Res 186:535–540

    Article  PubMed  CAS  Google Scholar 

  5. Wang YX, Rudnicki MA (2011) Satellite cells, the engines of muscle repair. Nat Rev Mol Cell Biol 13:127–133

    PubMed  Google Scholar 

  6. Pallafacchina G, Blaauw B, Schiaffino S (2012) Role of satellite cells in muscle growth and maintenance of muscle mass. Nutr Metab Cardiovasc Dis. [Epub ahead of print]

  7. Kao RL, Chiu RCJ (1997) Satellite cell implantation. In: Kao RL, Chiu RCJ (eds) Cellular cardiomyoplasty: myocardial repair with cell implantation. Chapman & Hall, New York, pp 129–162

    Google Scholar 

  8. Campion DR (1984) The muscle satellite cell: a review. Int Rev Cytol 87:225–251

    Article  PubMed  CAS  Google Scholar 

  9. Zhang M, McLennan IS (1994) Use of antibodies to identify satellite cells with a light microscope. Muscle Nerve 17:987–994

    Article  PubMed  CAS  Google Scholar 

  10. Kao RL (2001) Autologous satellite cell for myocardial regeneration. E-biomed: J Regen Med 2:1–8

    Article  Google Scholar 

  11. Mauro A (1961) Satellite cells of skeletal muscle fibers. J Biophys Biochem Cytol 9:493–495

    Article  PubMed  CAS  Google Scholar 

  12. Usas A, Mačiulaitis J, Mačiulaitis R et al (2011) Skeletal muscle-derived stem cells: implications for cell-mediated therapies. Medicina (Kaunas) 47:469–479

    Google Scholar 

  13. Aziz A, Sebastian S, Dilworth FJ (2012) The origin and fate of muscle satellite cells. Stem Cell Rev 8:609–622

    Article  PubMed  CAS  Google Scholar 

  14. Kao RL, Browder W, Li C (2009) Cellular cardiomyoplasty: what have we learned? Asian Cardiovasc Thorac Ann 17:89–101

    Article  PubMed  Google Scholar 

  15. Huu AL, Prakash S, Shum-Tim D (2012) Cellular cardiomyoplasty: current state of the field. Regen Med 7:571–582

    Article  PubMed  Google Scholar 

  16. Zhao R, Kao RL (2000) Comparison of labeling procedures for myogenic cells. Cardiac Vasc Regen 1:85–91

    Google Scholar 

  17. Al-Khaldi A, Lachapelle K, Galipeau J (2000) Endogenous β-galactosidase enzyme activities in normal tissue and ischemic myocardium. Cardiac Vasc Regen 1:283–290

    Google Scholar 

  18. Grajales L, García J, Geenen DL (2012) Induction of cardiac myogenic lineage development differs between mesenchymal and satellite cells and is accelerated by bone morphogenetic protein-4. J Mol Cell Cardiol 53:382–391

    Article  PubMed  CAS  Google Scholar 

  19. Danoviz ME, Yablonka-Reuveni Z (2012) Skeletal muscle satellite cells: background and methods for isolation and analysis in a primary culture system. Methods Mol Biol 798:21–52

    Article  PubMed  CAS  Google Scholar 

  20. Neal A, Boldrin L, Morgan JE (2012) The satellite cell in male and female, developing and adult mouse muscle: distinct stem cells for growth and regeneration. PLoS One 7(e37950):1–11

    Google Scholar 

  21. Di Foggia V, Robson L (2012) Isolation of satellite cells from single muscle fibers from young, aged, or dystrophic muscles. Methods Mol Biol 916:3–14

    Article  PubMed  Google Scholar 

  22. Yi Y, Noh MJ, Lee KH (2011) Current advances in retroviral gene therapy. Curr Gene Ther 11:218–228

    Article  PubMed  CAS  Google Scholar 

  23. Durand S, Cimarelli A (2011) The inside out of lentiviral vectors. Viruses 3:132–159

    Article  PubMed  CAS  Google Scholar 

  24. Sakuma T, Barry MA, Ikeda Y (2012) Lentiviral vectors: basic to translational. Biochem J 443:603–618

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgement

This work was supported by NIH grants HL072138, GM093878 and AHA grants 09GRNT2020111, 02555009B to RLK.

Author information

Authors and Affiliations

  1. Department of Surgery, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN, USA

    Grace W. Kao, Elizabeth K. Lamb & Race L. Kao

Authors
  1. Grace W. Kao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Elizabeth K. Lamb
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Race L. Kao
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. , Department of Surgery, East Tennessee State University, Johnson City, 37614, Tennessee, USA

    Race L. Kao

Rights and permissions

Reprints and permissions

Copyright information

© 2013 Springer Science+Business Media New York

About this protocol

Cite this protocol

Kao, G.W., Lamb, E.K., Kao, R.L. (2013). Skeletal Muscle Stem Cells. In: Kao, R. (eds) Cellular Cardiomyoplasty. Methods in Molecular Biology, vol 1036. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-511-8_2

Download citation

  • DOI: https://doi.org/10.1007/978-1-62703-511-8_2

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-510-1

  • Online ISBN: 978-1-62703-511-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation