Preparation and Characterization of DNA Block Copolymer Assemblies Loaded with Nanoparticles
We have recently developed a universal procedure to functionalize inorganic nanoparticles with a dense layer of DNA through the self-assembly of DNA block copolymers and nanoparticles. This functionalization strategy allows one to combine the useful physical properties of inorganic nanoparticle with the enhanced DNA binding properties that originate from the high surface DNA density. In particular, the hybrid nanostructures exhibit orders of magnitude higher binding constants than regular DNA strands. This chapter presents a detailed protocol for the preparation and characterization of DNA block copolymer assemblies loaded with nanoparticles.
Key wordsDNA Block copolymer Nanoparticles Self-assembly
Controlled pore glass
Dynamic light scattering
Block copolymer of DNA and polystyrene
Förster/Fluorescent Resonance Energy Transfer
DNA-b-polystyrene assemblies loaded with magnetic nanoparticles
Phosphate buffer saline
Polystyrene modified magnetic nanoparticles
Self assembly of DNA-b-polystyrene
Transmission electron microscope
This work was supported by the NSF Career Award (DMR-087646) and NSF-IGERT Fellowship (DGE-0221664).
- 14.Northwestern University. oligonucleotide properties calculator used to calculate extinction coefficient of DNA. http://www.basic.northwestern.edu/biotools/OligoCalc.html
- 16.Clegg RM, David M.J.L.a.J.E.D. (1992) Fluorescence resonance energy transfer and nucleic acids. Methods Enzymol, vol. 211, Academic, p 353–388Google Scholar
- 19.Zimmermann J, Kwak M, Musser AJ, Herrmann A (2011) Amphiphilic DNA block copolymers: nucleic acid-polymer hybrid materials for diagnostics and biomedicine. Methods Mol Biol 751:239–266Google Scholar