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Preparation and Characterization of DNA Block Copolymer Assemblies Loaded with Nanoparticles

  • Xi-Jun Chen
  • Robert J. Hickey
  • So-Jung Park
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1025)

Abstract

We have recently developed a universal procedure to functionalize inorganic nanoparticles with a dense layer of DNA through the self-assembly of DNA block copolymers and nanoparticles. This functionalization strategy allows one to combine the useful physical properties of inorganic nanoparticle with the enhanced DNA binding properties that originate from the high surface DNA density. In particular, the hybrid nanostructures exhibit orders of magnitude higher binding constants than regular DNA strands. This chapter presents a detailed protocol for the preparation and characterization of DNA block copolymer assemblies loaded with nanoparticles.

Key words

DNA Block copolymer Nanoparticles Self-assembly 

Abbreviations

CPG

Controlled pore glass

DLS

Dynamic light scattering

DMF

N,N-Dimethylformamide

DNA-b-PS

Block copolymer of DNA and polystyrene

FAM

Fluorescein

FRET

Förster/Fluorescent Resonance Energy Transfer

MNP

Magnetic nanoparticles

MNP@PS@DNA

DNA-b-polystyrene assemblies loaded with magnetic nanoparticles

PBS

Phosphate buffer saline

PS

Polystyrene

PS-MNP

Polystyrene modified magnetic nanoparticles

PS@DNA

Self assembly of DNA-b-polystyrene

PTFE

Polytetrafluoroethylene

TEM

Transmission electron microscope

THF

Tetrahydrofuran

Notes

Acknowledgments

This work was supported by the NSF Career Award (DMR-087646) and NSF-IGERT Fellowship (DGE-0221664).

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Xi-Jun Chen
    • 1
  • Robert J. Hickey
    • 1
  • So-Jung Park
    • 1
  1. 1.Department of ChemistryUniversity of PennsylvaniaPhiladelphiaUSA

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