Abstract
Fast isolation by AFLP of sequences containing repeats (FIASCO) is a rapid and simple method for separating microsatellite-containing DNA fragments from genomic DNA de novo. The method takes the advantage of the amplified fragment length polymorphism (AFLP) technique that relies on effective digestion–ligation reaction. The repeat-containing fragments are selectively hybridized to biotinylated probes and harvested by streptavidin-coated magnetic beads. The enriched microsatellite-containing fragments can be cloned and sequenced to yield a variety of microsatellite loci for applications in many different fields in molecular genetics.
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Acknowledgments
The work was supported by the Centre of Excellence in Evolutionary Research of the Academy of Finland and the Finnish Cultural Foundation. Alessandro Grapputo, Elina Virtanen, and Sari Viinikainen provided valuable comments on the method and on the manuscript.
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Rikalainen, K. (2013). Fast Isolation by AFLP of Sequences Containing Repeats. In: Kantartzi, S. (eds) Microsatellites. Methods in Molecular Biology, vol 1006. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-389-3_4
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DOI: https://doi.org/10.1007/978-1-62703-389-3_4
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