Abstract
Sample preparation and laser-induced fluorescence detection are two key steps of the analytical methodology to determine by capillary electrophoresis low concentrations of proteins in complex sample matrices. In this chapter the options of performing both steps in different ways are shown by detailing the analysis of the allergen β-lactoglobulin in food products for infants and the analysis of the isoforms of alpha 1-acid glycoprotein, a potential biomarker, in serum and secretome.
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Acknowledgments
Isabel Vasallo from Hero Institute for Infant Nutrition (Hero España, S.A., Murcia, Spain) is acknowledged for preparing the infant food samples. Financial support from the Spanish Ministry of Science and Innovation (project CTQ2009-09399), CSIC (project 2010JP0013), and the Hungarian Government (project OTKA K-81839) is acknowledged. Angel Puerta acknowledges CSIC for a contract in the JAE doc program cofinanced by the European Union under the European Social Fund, and Raul Garrido-Medina acknowledges the Spanish Ministry of Education the Ph.D. fellowship (FPU) awarded.
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Garrido-Medina, R. et al. (2013). Capillary Electrophoresis with Laser-Induced Fluorescence Detection of Proteins from Two Types of Complex Sample Matrices: Food and Biological Fluids. In: Volpi, N., Maccari, F. (eds) Capillary Electrophoresis of Biomolecules. Methods in Molecular Biology, vol 984. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-296-4_15
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DOI: https://doi.org/10.1007/978-1-62703-296-4_15
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