Abstract
Variations in the amino acid sequence, glycosylation, and/or other posttranslational modifications in glycoproteins give rise to different molecules of the glycoprotein called forms. Qualitative and/or quantitative alterations in these forms are related to pathophysiological situations in the individuals. In this study, a methodology to analyze these differences in forms of the alpha 1-acid glycoprotein (AGP) between healthy individuals and patients with two different vascular diseases is detailed. The whole methodology includes a sample preparation method based on immunochromatography, a capillary electrophoresis method for separation of AGP peaks (isoforms), and statistical methods (Linear Discriminant Analysis) for sample classification. As a result, it is shown that the methodology proposed allows studying the role of AGP isoforms as potential vascular disease biomarkers.
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Acknowledgements
Financial support from Comunidad de Madrid (project S-GEN/0247/2006) and the Spanish Ministry of Science and Innovation (project CTQ2009-09399) is acknowledged. Angel Puerta acknowledges the Spanish National Research Council (CSIC) for a contract in the JAEdoc program. Samples of plasma from patients with AAA, stable CTA, and healthy donors matched in age and sex were provided by Fundación Jiménez Díaz (Madrid, Spain).
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Puerta, A., Martin-Alvarez, P.J., Ongay, S., Diez-Masa, J.C., de Frutos, M. (2013). Immunoaffinity, Capillary Electrophoresis, and Statistics for Studying Intact Alpha 1-Acid Glycoprotein Isoforms as an Atherothrombosis Biomarker. In: Phillips, T., Kalish, H. (eds) Clinical Applications of Capillary Electrophoresis. Methods in Molecular Biology, vol 919. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-029-8_20
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DOI: https://doi.org/10.1007/978-1-62703-029-8_20
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