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Retrovirus-Based mRNA Transfer for Transient Cell Manipulation

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Synthetic Messenger RNA and Cell Metabolism Modulation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 969))

Abstract

Retrovirus-mediated mRNA transfer (RMT) combines the advantageous features of retrovirus-mediated cell targeting and entry with the controlled transfer of mRNAs. We have recently exploited this strategy for the dose-controlled transfer of recombinases and DNA transposases, avoiding cytotoxicity and potential insertional mutagenesis. Further applications can be envisaged, especially when low expression levels are sufficient to modify cell fate or function. Here we describe a step-by-step protocol for the generation of RMT vector particles, their titration and their application in a model cell line.

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Acknowledgments

This work was supported by grants by the Deutsche Forschungs-gemeinschaft (SFB738 project C4; excellence cluster REBIRTH), the German Ministry for Research and Education (BMBF), and the European Union (FP7 project PERSIST). We thank Ivonne Fernandez, Girmay Asgedom, and Thomas Neumann for technical assistance and Tamaryin Godinho for critically reading the manuscript.

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Authors and Affiliations

  1. Hannover Medical School, Institute of Experimental Hematology, Hannover, Germany

    Melanie Galla, Axel Schambach & Christopher Baum

Authors
  1. Melanie Galla
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  2. Axel Schambach
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  3. Christopher Baum
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Corresponding author

Correspondence to Christopher Baum .

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Editors and Affiliations

  1. The Anlyan Center, Department of Genetics, Yale School of Medicine, Cedar Street 300, New Haven, 06520-8005, Connecticut, USA

    Peter M. Rabinovich

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Galla, M., Schambach, A., Baum, C. (2013). Retrovirus-Based mRNA Transfer for Transient Cell Manipulation. In: Rabinovich, P. (eds) Synthetic Messenger RNA and Cell Metabolism Modulation. Methods in Molecular Biology, vol 969. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-260-5_10

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  • DOI: https://doi.org/10.1007/978-1-62703-260-5_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-259-9

  • Online ISBN: 978-1-62703-260-5

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