Synthetic Messenger RNA and Cell Metabolism Modulation

Methods and Protocols

  • Peter M. Rabinovich

Part of the Methods in Molecular Biology book series (MIMB, volume 969)

Table of contents

  1. Front Matter
    Pages i-xi
  2. mRNA Synthesis, Purification, and Transfer

    1. Front Matter
      Pages 1-1
    2. Peter M. Rabinovich, Sherman M. Weissman
      Pages 3-28
    3. Norbert Pardi, Hiromi Muramatsu, Drew Weissman, Katalin Karikó
      Pages 29-42
    4. Drew Weissman, Norbert Pardi, Hiro Muramatsu, Katalin Karikó
      Pages 43-54
    5. Ewa Grudzien-Nogalska, Joanna Kowalska, Wei Su, Andreas N. Kuhn, Sergey V. Slepenkov, Edward Darzynkiewicz et al.
      Pages 55-72
    6. Sigurd Leinæs Bøe, Eivind Hovig
      Pages 89-100
    7. Jaewoo Lee, David Boczkowski, Smita Nair
      Pages 101-110
    8. Jaewoo Lee, David Boczkowski, Smita Nair
      Pages 111-125
    9. Linhong Li, Cornell Allen, Rama Shivakumar, Madhusudan V. Peshwa
      Pages 127-138
    10. Melanie Galla, Axel Schambach, Christopher Baum
      Pages 139-161
  3. Modulation and Monitoring of Cell Metabolism

    1. Front Matter
      Pages 185-185
    2. Tobias Riet, Astrid Holzinger, Jan Dörrie, Niels Schaft, Gerold Schuler, Hinrich Abken
      Pages 187-201
    3. Noriko Shimasaki, Dario Campana
      Pages 203-220
    4. Mustafa Diken, Sebastian Kreiter, Abderraouf Selmi, Özlem Türeci, Ugur Sahin
      Pages 235-246

About this book

Introduction

Synthetic mRNA is an attractive tool for mammalian cell reprogramming that  can be used in basic research, as well as in clinical applications. Present mRNA in vitro synthesis is a rather simple procedure, which delivers a high yield of quality product. Various modifications may be introduced into the mRNA by changing  the sequence of the DNA template, by modifying the reaction of transcription, or by post-transcriptional modification. mRNA, as a transfection agent, has several advantages over DNA, as mRNA expression is not dependent on nuclear entry and occurs directly in the cytosol. Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols covers the typical main methods, such as mRNA synthesis, modifications, and delivery.  Examples of cell reprogramming and analysis in the fields of immunotherapy and stem cell research are also included. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols will be of interest to researchers, clinicians, and biotech companies interested in mRNA-mediated cell reprogramming.

Keywords

cell metabolism cell reprogramming immunotherapy mRNA synthesis synthetic messenger RNA transfection agent

Editors and affiliations

  • Peter M. Rabinovich
    • 1
  1. 1.The Anlyan Center, Department of GeneticsYale School of MedicineNew HavenUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-62703-260-5
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-259-9
  • Online ISBN 978-1-62703-260-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book