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p53 Actions on MicroRNA Expression and Maturation Pathway

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p53 Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 962))

Abstract

The tumor suppressor p53 orchestrates multiple cellular pathways as a central node of anti-oncogenic programs in response to DNA damage, oncogene activation, and several stresses. In addition to the principal role as a transcription factor that transactivates many target genes involved in apoptosis and cell cycle control, p53 has been shown to exert various transactivation-independent effects both in the nucleus and in the cytoplasm. Diversity of p53 activities is further emphasized by the recent studies revealing the close interaction between the p53 and microRNA (miRNA) world. We recently demonstrated that p53 promotes the processing of several primary miRNA transcripts through association with Drosha, a central RNase III in miRNA biogenesis, under DNA damage-inducing conditions. In contrast to wild-type p53, cancer-derived p53 mutants attenuate miRNA maturation. These findings reveal a novel aspect of p53 activities and suggest complex crosstalks between miRNA biogenesis and intracellular signaling pathways. In this chapter, we describe the methods for evaluation of the effects of p53 on miRNA expression, an interaction between pri-miRNA and Drosha complex, and pri-miRNA processing activity of the Drosha complex.

Potential conflicts of interest: none.

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Acknowledgments

We thank K. Yamagata and S. Kato for technical advice on in vitro processing analysis. This work was supported by KAKENHI (Grant-in-Aid for Scientific Research) and the Global Center of Excellence Program for “Integrative Life Science Based on the Study of Biosignaling Mechanisms” from the Ministry of Education, Culture, Sports, Science, and Technology of Japan. H.I.S. is supported by a research fellowship of the Japan Society for the Promotion of Science for Young Scientists.

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Authors and Affiliations

  1. Department of Molecular Pathology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan

    Hiroshi I. Suzuki & Kohei Miyazono

Authors
  1. Hiroshi I. Suzuki
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  2. Kohei Miyazono
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Corresponding author

Correspondence to Kohei Miyazono .

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Editors and Affiliations

  1. Massey Cancer Center, Department of Biochemistry, Virginia Commonwealth University, College Street 401, Richmond, 23298, Virginia, USA

    Sumitra Deb

  2. Massey Cancer Center, Department of Biochemistry, Virginia Commonwealth University, College Street 401, Richmond, 23298, Virginia, USA

    Swati Palit Deb

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Suzuki, H.I., Miyazono, K. (2013). p53 Actions on MicroRNA Expression and Maturation Pathway. In: Deb, S., Deb, S. (eds) p53 Protocols. Methods in Molecular Biology, vol 962. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-236-0_14

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  • DOI: https://doi.org/10.1007/978-1-62703-236-0_14

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-235-3

  • Online ISBN: 978-1-62703-236-0

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