p53 Protocols

  • Sumitra Deb
  • Swati Palit Deb

Part of the Methods in Molecular Biology book series (MIMB, volume 962)

Table of contents

  1. Front Matter
    Pages i-x
  2. Marie P. Khoury, Virginie Marcel, Kenneth Fernandes, Alexandra Diot, David P. Lane, Jean-Christophe Bourdon
    Pages 1-14
  3. Virginie Marcel, Marie P. Khoury, Kenneth Fernandes, Alexandra Diot, David P. Lane, Jean-Christophe Bourdon
    Pages 15-29
  4. Rachel W. Goehe, Molly L. Bristol, Eden N. Wilson, David A. Gewirtz
    Pages 31-48
  5. Emir Senturk, James J. Manfredi
    Pages 49-61
  6. Ian M. Love, Dingding Shi, Steven R. Grossman
    Pages 63-73
  7. Angelina V. Vaseva, Ute M. Moll
    Pages 75-84
  8. Emir Senturk, James J. Manfredi
    Pages 113-125
  9. Mahesh Ramamoorthy, Catherine Vaughan, Sumitra Deb, Swati Palit Deb
    Pages 127-133
  10. W. Andrew Yeudall, Katharine H. Wrighton, Sumitra Deb
    Pages 135-146
  11. Hiroshi I. Suzuki, Kohei Miyazono
    Pages 165-181
  12. Venkat S. Gadepalli, Catherine Vaughan, Raj R. Rao
    Pages 183-191
  13. Catherine Vaughan, Swati Palit Deb, Sumitra Deb
    Pages 193-199
  14. Jun-Ming Liao, Hua Lu
    Pages 201-210
  15. Frauke Goeman, Giulia Fontemaggi, Giovanni Blandino
    Pages 211-226
  16. Catherine Vaughan, Brad Windle, Sumitra Deb
    Pages 227-236

About this book


The second edition of p53 Protocols expands upon the first edition with progress in p53 research since the publication of the first edition. In p53 Protocols, Second Edition expert researchers in the field detail many of the methods that address the challenging questions of the p53 field.  These methods include the identification of the target genes and binding partners of gain of function p53 mutants, methods to determine stress response, autophagy or senescence induced by p53, cell cycle analysis, analysis of different phases of DNA replication modified by p53, and generation of pluripotent stem cells.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, p53 Protocols, Second Edition seeks to aid scientists in the further study into p53 and other tumor suppressors.


DNA replication autophagy binding partners pluripotent stem cells senescence stress response target genes tumor suppressors

Editors and affiliations

  • Sumitra Deb
    • 1
  • Swati Palit Deb
    • 2
  1. 1.Massey Cancer Center, Department of BiochemistryVirginia Commonwealth UniversityRichmondUSA
  2. 2.Massey Cancer Center, Department of BiochemistryVirginia Commonwealth UniversityRichmondUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-62703-236-0
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-235-3
  • Online ISBN 978-1-62703-236-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book