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Production of Pathogen-Free Horticultural Crops by Cryotherapy of In Vitro-Grown Shoot Tips

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Protocols for Micropropagation of Selected Economically-Important Horticultural Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 994))

Abstract

Horticultural crops are economically valuable for sustainable agricultural production. Plant diseases caused by Pathogens including virus, phytoplasma and bacterium have been a great threat to production of horticultural crops. The efficient use of pathogen-free plant materials has overcome the menace of plant diseases and has sustained crop production. Cryotherapy of shoot tips, a novel application of cryopreservation technique, has become a new plant biotechnology tool for plant pathogen eradication. When compared with the traditional methods, cryotherapy of shoot tips produces high frequency of pathogen-free plants, which is independent of shoot tip size and cryogenic methods. Cryotherapy of shoot tips has six major steps to produce pathogen-free plants: (1) introduction of infected plant materials into in vitro cultures; (2) excision of shoot tips; (3) cryotherapy; (4) post-culture for plant regeneration; (5) indexing of pathogens in regenerated plants after cryotherapy; and (6) establishment of pathogen-free nuclear stock plants. The key steps 2, 3, and 4 are similar to cryopreservation, and play a major role in obtaining high pathogen eradication frequency.

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Acknowledgments

The present study was financially supported by the Key Project “13115” of Shaanxi Province (project No. 2009ZDKG-10) and President Foundation of Northwest A & F University.

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Authors and Affiliations

  1. Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Horticulture, Northwest Agriculture and Forestry University, Shaanxi, China

    Chaohong Feng, Renrui Wang, Jingwei Li, Biao Wang, Zhenfang Yin, Zhenhua Cui, Baiquan Li, Wenlu Bi, Zhibo Zhang & Qiaochun Wang

  2. Institute of Animal and Plant Quarantine, Chinese Academy of Inspection and Quarantine (CAIQ), Beijing, China

    Mingfu Li

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  1. Chaohong Feng
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  2. Renrui Wang
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  3. Jingwei Li
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  5. Zhenfang Yin
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  6. Zhenhua Cui
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Correspondence to Qiaochun Wang .

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Editors and Affiliations

  1. , IVALSA/Istituto per la Valorizzazione, National Research Council (CNR), Polo Scientifico, via Madonna del Piano n. 10, Sesto Fiorentino, Florence, 50019, Italy

    Maurizio Lambardi

  2. , Istituto per la Valorizzazione del Legno, National Research Council (CNR), n/a, Florence, n/a, Italy

    Elif Aylin Ozudogru

  3. , Department of Agricultural, University of Helsinki, 5 Latokartanonkaari Room C 130, Helsinki, 00014, Finland

    Shri Mohan Jain

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Feng, C. et al. (2012). Production of Pathogen-Free Horticultural Crops by Cryotherapy of In Vitro-Grown Shoot Tips. In: Lambardi, M., Ozudogru, E., Jain, S. (eds) Protocols for Micropropagation of Selected Economically-Important Horticultural Plants. Methods in Molecular Biology, vol 994. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-074-8_35

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  • DOI: https://doi.org/10.1007/978-1-62703-074-8_35

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-073-1

  • Online ISBN: 978-1-62703-074-8

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