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Cryopreservation of in vitro-grown shoot tips of raspberry (Rubus idaeus L.) by encapsulation–vitrification and encapsulation–dehydration

  • Cell Biology and Morphogenesis
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Abstract

The first efficient cryopreservation procedure for in vitro-grown shoot tips of raspberry (Rubus idaeus L.) has been developed based on encapsulation–vitrification (EnVi) and encapsulation–dehydration (EnDe). EnVi resulted in higher survival (85%) and regrowth (75%) of cryopreserved shoot tips than EnDe (65 and 50%, respectively). In both cryogenic procedures, shoots regenerated from cryopreserved shoot tips without intermediary callus formation. Histological studies showed that a much larger number of meristematic cells survived following EnVi than EnDe. The EnVi procedure was applied to seven raspberry genotypes with an average survival and regrowth of 71 and 68%, respectively. Regenerated plants showed normal morphology. Results here indicate EnVi as a simple and efficient method for long-term preservation of R. idaeus germplasm.

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Fig. 1a–f
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Fig. 6a–c
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Abbreviations

AC::

Activated charcoal

BAP::

Benzylaminopurine

BM::

Basic medium

DMSO::

Dimethylsulphoxide

IBA::

Indol-3-yl-acetic acid

EnDe::

Encapsulation–dehydration

EnVi::

Encapsulation–vitrification

LN::

Liquid nitrogen

MS::

Murashige and Skoog (1962) medium

PVP::

Polyvinylpyrrolidone

PVS2::

Plant vitrification solution 2

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Acknowledgements

Financial support from the University of Helsinki and the Ministry of Agriculture and Forestry, Finland, is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Applied Biology, University of Helsinki, P.O. Box 27, 00014, Helsinki, Finland

    Qiaochun Wang & Jari P. T. Valkonen

  2. AgriFood Research Centre, Laukaa Research and Elite Plant Unit, Antinniementie 1, 41330, Vihtavuori, Finland

    Jaana Laamanen & Marjatta Uosukainen

  3. Present address: Institute of Horticulture, Sichuan Academy of Agricultural Science, Chengdu, 610016, Sichuan, P. R. China

    Qiaochun Wang

Authors
  1. Qiaochun Wang
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  2. Jaana Laamanen
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  3. Marjatta Uosukainen
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  4. Jari P. T. Valkonen
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Correspondence to Jari P. T. Valkonen.

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Communicated by H. Lörz

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Wang, Q., Laamanen, J., Uosukainen, M. et al. Cryopreservation of in vitro-grown shoot tips of raspberry (Rubus idaeus L.) by encapsulation–vitrification and encapsulation–dehydration. Plant Cell Rep 24, 280–288 (2005). https://doi.org/10.1007/s00299-005-0936-x

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  • DOI: https://doi.org/10.1007/s00299-005-0936-x

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