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Utilizing AntagomiR (Antisense microRNA) to Knock Down microRNA in Murine Bone Marrow Cells

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Rational Drug Design

Part of the book series: Methods in Molecular Biology ((MIMB,volume 928))

Abstract

MicroRNAs (miRNAs) are highly conserved small RNAs which regulate gene expression primarily through base pairing to the 3′ untranslated region of target messenger RNA (mRNA), leading to mRNA degradation or translation inhibition depending on the complementarity between the miRNA and target mRNA. Single miRNA regulates multiple target mRNA. miRNAs have been shown to regulate gene expression in the hematopoietic stem cells, as well as at key decision points for various lineages. However, aberrant expression of miRNAs has been documented in cancer and disease models. Rigorous dissection of miRNA pathways and biology requires facile loss of function modeling. This chapter describes detailed protocol for knockdown miRNA-21 which is involved in myelopoiesis using antagomiRs in primary murine bone marrow stem/progenitor cells.

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Acknowledgments

This work was partially supported by grants from Alex’s Lemonade Stand, and NIH R21 CA142601 and R01 CA159845 (H.L.G.).

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Authors and Affiliations

  1. Immunobiology Division, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, USA

    Chinavenmeni S. Velu & H. Leighton Grimes

Authors
  1. Chinavenmeni S. Velu
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  2. H. Leighton Grimes
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Corresponding author

Correspondence to H. Leighton Grimes .

Editor information

Editors and Affiliations

  1. Research Foundation, Div. Experimental Hematology, Cincinnati Children's Hospital, Burnet Ave. 3333, Cincinnati, 45229, Ohio, USA

    Yi Zheng

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© 2012 Springer Science+Business Media New York

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Velu, C.S., Grimes, H.L. (2012). Utilizing AntagomiR (Antisense microRNA) to Knock Down microRNA in Murine Bone Marrow Cells. In: Zheng, Y. (eds) Rational Drug Design. Methods in Molecular Biology, vol 928. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-008-3_15

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  • DOI: https://doi.org/10.1007/978-1-62703-008-3_15

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-007-6

  • Online ISBN: 978-1-62703-008-3

  • eBook Packages: Springer Protocols

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