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Isolating Genes Involved with Genotoxic Drug Response in the Nematode Caenorhabditis elegans Using Genome-Wide RNAi Screening

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DNA Repair Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 920))

Abstract

The soil nematode Caenorhabditis elegans has become a popular genetic model organism used to study a broad range of complex biological processes, including development, aging, apoptosis, and DNA damage responses. Many genetic tools and tricks have been developed in C. elegans including knock down of gene expression via RNA interference (RNAi). In C. elegans RNAi can effectively be administrated via feeding the nematodes bacteria expressing double-stranded RNA targeting the gene of interest. Several commercial C. elegans RNAi libraries are available and hence gene inactivation using RNAi can relatively easily be performed in a genome-wide fashion. In this chapter we give a protocol for using genome-wide RNAi screening to identify genes involved with the response to genotoxic stress.

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, University of Aarhus, Aarhus, Denmark

    Lone Vedel Schøler, Tine Hørning Møller, Steffen Nørgaard, Lotte Vestergaard & Anders Olsen

Authors
  1. Lone Vedel Schøler
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  2. Tine Hørning Møller
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  3. Steffen Nørgaard
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  4. Lotte Vestergaard
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  5. Anders Olsen
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Corresponding author

Correspondence to Anders Olsen .

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Editors and Affiliations

  1. , Department of Molecular Biology, Aarhus University, C.F Møllers Allé bygning 1130, Århus C, 8000, Denmark

    Lotte Bjergbæk

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Schøler, L.V., Møller, T.H., Nørgaard, S., Vestergaard, L., Olsen, A. (2012). Isolating Genes Involved with Genotoxic Drug Response in the Nematode Caenorhabditis elegans Using Genome-Wide RNAi Screening. In: Bjergbæk, L. (eds) DNA Repair Protocols. Methods in Molecular Biology, vol 920. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-998-3_3

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  • DOI: https://doi.org/10.1007/978-1-61779-998-3_3

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-997-6

  • Online ISBN: 978-1-61779-998-3

  • eBook Packages: Springer Protocols

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