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Bioprospecting Metagenomics for New Glycoside Hydrolases

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Biomass Conversion

Part of the book series: Methods in Molecular Biology ((MIMB,volume 908))

Abstract

To efficiently deconstruct recalcitrant plant biomass to fermentable sugars in industrial processes, biocatalysts of higher performance and lower cost are required. The genetic diversity found in the metagenomes of natural microbial biomass decay communities may harbor such enzymes. The aim of this chapter is to describe strategies, based on metagenomic approaches, for the discovery of glycoside hydrolases (GHases) from microbial biomass decay communities, especially those from unknown or never-been-cultivated microorganisms.

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Acknowledgments

The authors wish to acknowledge a subcontract from the BioEnergy Science Center, which is a US Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science.

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Authors and Affiliations

  1. Department of Ecology & Evolution, University of Chicago, Chicago, IL, USA

    Jack Gilbert

  2. Biology Department, Brookhaven National Laboratory, Upton, NY, USA

    Luen-Luen Li & Sean M. McCorkle

  3. Center for Agriculture and Environmental Biotechnology, RTI International, Research Triangle Park, NC, USA

    Safiyh Taghavi & Daniel van der Lelie

  4. DOE Joint Genome Institute, Walnut Creek, CA, USA

    Susannah Tringe

Authors
  1. Jack Gilbert
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  2. Luen-Luen Li
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  3. Safiyh Taghavi
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  4. Sean M. McCorkle
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  5. Susannah Tringe
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  6. Daniel van der Lelie
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Corresponding author

Correspondence to Daniel van der Lelie .

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Editors and Affiliations

  1. , Biosciences Center, National Renewable Energy Laboratory, Cole Boulevard 1617, Golden, 80401-3305, USA

    Michael E. Himmel

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Gilbert, J., Li, LL., Taghavi, S., McCorkle, S.M., Tringe, S., van der Lelie, D. (2012). Bioprospecting Metagenomics for New Glycoside Hydrolases. In: Himmel, M. (eds) Biomass Conversion. Methods in Molecular Biology, vol 908. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-956-3_14

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  • DOI: https://doi.org/10.1007/978-1-61779-956-3_14

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-955-6

  • Online ISBN: 978-1-61779-956-3

  • eBook Packages: Springer Protocols

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