Abstract
Mesenchymal stem cells (MSCs) are multipotent cells with the potential to differentiate into bone, cartilage, adipose, and muscle cells. Adult derived MSCs are being actively investigated because of their potential to be utilized for therapeutic cell-based transplantation. Methods to track MSCs in vivo are limited, preventing long-term functional studies of transplanted cells. Quantum Dots (QDs) offer an alternative to organic dyes and fluorescent proteins to label and track cells in vitro and in vivo. Nanoparticles are resistant to chemical and metabolic degradation, demonstrating long-term photostability. Here, we describe the technique to label MSCs with QDs and demonstrate intracellular QD distribution in the labeled MSCs with laser scanning confocal fluorescent microscopy.
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References
Jaiswal J, Mattoussi H, Mauro J, Simon S (2003) Long-term multiple color imaging of live cells using quantum dot bioconjugates. Nat Biotechnol 21:47–51
Chan W, Maxwell D, Gao X, Bailey R, Han M (2002) Luminescent quantum dots for multiplexed biological detection and imaging. Curr Opin Biotechnol 13:40–46
Alvisiatos A (2004) The use of nanocrystals in biological detection. Nat Biotechnol 22:47–52
Jaiswal JK, Simon SM (2004) Potentials and pitfalls of fluorescent quantum dots for biological imaging. Trends Cell Biol 14(9):497–504
Zheng J, Ghazani A, Song Q, Mardvani S, Chan W, Wang C (2006) Cellular imaging and surface marker labeling of hematopoietic cells using quantum dot bioconjugates. Lab Hematol 12:94–98
Muller-Borer BJ, Collins MC, Gunst PR, Cascio WE, Kypson AP (2007) Quantum dot labeling of mesenchymal stem cells. J Nanobiotechnolgy 5:1–9
Rosen A, Kelly D, Schuldt A, Potapova I, Doronin K, Robichaud R, Rosen M, Brink P, Gaudette G, Cohen I (2007) Finding fluorescent needles in the cardiac haystack: tracking human mesenchymal stem cells labeled with quantum dots for quantitative in vivo 3-D fluorescence analysis. Stem Cells 25:2128–2138
Jaiswal J, Goldman E, Mattoussi H, Simo S (2004) Use of quantum dots for live cell imaging. Nat Methods 1(1):73–78
Larson D, Zipfel W, Williams R, Clark S, Bruchez M, Wise F, Webb W (2004) Water-soluble quantum dots for multiphoton fluorescence imaging in vivo. Science 300:1434–1436
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Collins, M.C., Gunst, P.R., Cascio, W.E., Kypson, A.P., Muller-Borer, B.J. (2012). Labeling and Imaging Mesenchymal Stem Cells with Quantum Dots. In: Soloviev, M. (eds) Nanoparticles in Biology and Medicine. Methods in Molecular Biology, vol 906. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-953-2_15
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DOI: https://doi.org/10.1007/978-1-61779-953-2_15
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-952-5
Online ISBN: 978-1-61779-953-2
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