Abstract
Neurons cultured from rodent central nervous system tissues represent an important tool in the study of neurodegenerative disease mechanisms and neuroregenerative processes, including the survival- and axon growth–promoting properties of neurotrophic factors. This chapter presents a detailed protocol for the preparation of rat and mouse cortical and hippocampal neuron cell cultures using either embryonic or postnatal tissue with enzymatic digestion.
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Acknowledgment
L. Facci was supported by Fondazione CARIPARO “Progetto Dottorati di Ricerca” Anno 2009.
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© 2012 Springer Science+Business Media, LLC
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Facci, L., Skaper, S.D. (2012). Culture of Rodent Cortical and Hippocampal Neurons. In: Skaper, S. (eds) Neurotrophic Factors. Methods in Molecular Biology, vol 846. Humana Press. https://doi.org/10.1007/978-1-61779-536-7_5
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DOI: https://doi.org/10.1007/978-1-61779-536-7_5
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Online ISBN: 978-1-61779-536-7
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