Neurotrophic Factors

Methods and Protocols

  • Stephen D. Skaper

Part of the Methods in Molecular Biology book series (MIMB, volume 846)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Laura Facci, Stephen D. Skaper
    Pages 49-56
  3. Stephen D. Skaper, Carla Argentini, Massimo Barbierato
    Pages 67-77
  4. Stephen D. Skaper, Laura Facci
    Pages 79-89
  5. Stephen D. Skaper, Giulia Mercanti, Laura Facci
    Pages 91-101
  6. Jose V. Montoya-Gacharna, Jhon Jairo Sutachan, Wai Si Chan, Alexandra Sideris, Thomas J. J. Blanck, Esperanza Recio-Pinto
    Pages 103-116
  7. Stephen D. Skaper
    Pages 117-129
  8. Stephen D. Skaper
    Pages 159-166

About this book


Nervous system development evolves from the well-orchestrated processes of neural induction, cell proliferation, differentiation, cell migration, survival, and synapse formation. Among these environmental cues, neurotrophic factors are secreted proteins that promote neurite outgrowth, neuronal cell differentiation and survival both in vivo and in vitro. Nerve growth factor (NGF) is the founding and best characterized member of the neurotrophin family of neurotrophic polypeptides. Neurotrophic Factors: Methods and Protocols presents a selection of protocols and procedures which make use of cellular, tissue, and whole animal models which can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. Chapters cover a wide-range of topics such as dealing with the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia co-culture models, and cell-based assays for the evaluation of neuroprotective molecules, as well as assays which can be applied to the study of agents with neuroregenerative potential. Protocols describing viral- and nanoparticle-based delivery methods to neural cells are also presented, following by chapters dealing with organotypic slice culture protocols. Lastly, several chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Neurotrophic Factors: Methods and Protocols seeks to serve both professionals and novices alike with its well-honed methodologies in an effort to further our knowledge of what has been described as the last frontier of science.


nerve growth factor (NGF) neural cells neurite outgrowth neurobiological research neuronal cell differentiation neurotrophic polypeptides

Editors and affiliations

  • Stephen D. Skaper
    • 1
  1. 1.Dipto. Farmacologia e AnestesiologiaUniversita PadovaPadovaItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-535-0
  • Online ISBN 978-1-61779-536-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book