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Mapping Protein–DNA and Protein–Protein Interactions of ATP-Dependent Chromatin Remodelers

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Transcriptional Regulation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 809))

Abstract

Chromatin plays a key regulatory role in several DNA-dependent processes as it regulates DNA access to different protein factors. Several multisubunit protein complexes interact, modify, or mobilize nucleo-somes: the basic unit of chromatin, from its original location in an ATP-dependent manner to facilitate processes, such as transcription, replication, repair, and recombination. Knowledge of the interactions of chromatin remodelers with nucleosomes is a crucial requirement to understand the mechanism of chromatin remodeling. Here, we describe several methods to analyze the interactions of multisubunit chromatin-remodeling enzymes with nucleosomes.

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Authors and Affiliations

  1. Southern Illinois University School of Medicine, Carbondale, IL, USA

    Swetansu K. Hota, Mekonnen Lemma Dechassa, Punit Prasad & Blaine Bartholomew

Authors
  1. Swetansu K. Hota
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  2. Mekonnen Lemma Dechassa
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  3. Punit Prasad
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  4. Blaine Bartholomew
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Corresponding author

Correspondence to Blaine Bartholomew .

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Editors and Affiliations

  1. , Department of Biological Sciences, St. John's University, Utopia Parkway 8000, Queens, 11439, New York, USA

    Ales Vancura

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Hota, S.K., Dechassa, M.L., Prasad, P., Bartholomew, B. (2012). Mapping Protein–DNA and Protein–Protein Interactions of ATP-Dependent Chromatin Remodelers. In: Vancura, A. (eds) Transcriptional Regulation. Methods in Molecular Biology, vol 809. Springer, New York, NY. https://doi.org/10.1007/978-1-61779-376-9_26

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  • DOI: https://doi.org/10.1007/978-1-61779-376-9_26

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-61779-375-2

  • Online ISBN: 978-1-61779-376-9

  • eBook Packages: Springer Protocols

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