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Methods for Studying the G2 DNA Damage Checkpoint in Mammalian Cells

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Cell Cycle Checkpoints

Part of the book series: Methods in Molecular Biology ((MIMB,volume 782))

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Abstract

In response to post-replicative DNA damage, cells activate the G2 DNA damage checkpoint to ensure mitosis is not attempted until the damage has been repaired. This is a common response to a variety of DNA damaging agents, including ionizing radiation and many chemotherapeutic agents used in the treatment of cancer. The G2 DNA damage checkpoint acts to inhibit the mitotic cyclin-dependent kinase, and thus cells are arrested in the G2 phase of the cell cycle. The kinetics of this checkpoint can be assayed by staining cells for markers of mitosis, which can then be quantified by flow cytometry or microscopy.

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Acknowledgments

This work was supported by NIH grant GM087326.

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Authors and Affiliations

  1. Department of Oncological Sciences, Mount Sinai School of Medicine, New York, NY, 10029, USA

    Claudia Tapia-Alveal & Matthew J. O’Connell

Authors
  1. Claudia Tapia-Alveal
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  2. Matthew J. O’Connell
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Corresponding author

Correspondence to Matthew J. O’Connell .

Editor information

Editors and Affiliations

  1. , Department of Medicine, University of California, Gilman Drive #0063 9500, La Jolla, 92093-0063, California, USA

    Willis X. Li

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Tapia-Alveal, C., O’Connell, M.J. (2011). Methods for Studying the G2 DNA Damage Checkpoint in Mammalian Cells. In: Li, W. (eds) Cell Cycle Checkpoints. Methods in Molecular Biology, vol 782. Humana Press. https://doi.org/10.1007/978-1-61779-273-1_3

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  • DOI: https://doi.org/10.1007/978-1-61779-273-1_3

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-272-4

  • Online ISBN: 978-1-61779-273-1

  • eBook Packages: Springer Protocols

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