Cell Cycle Checkpoints

Methods and Protocols

  • Willis X. Li

Part of the Methods in Molecular Biology book series (MIMB, volume 782)

Table of contents

  1. Front Matter
    Pages i-x
  2. Claudia Tapia-Alveal, Matthew J. O’Connell
    Pages 23-31
  3. Katarzyna Siudeja, Jannie de Jong, Ody C.M. Sibon
    Pages 59-73
  4. Seetha V. Srinivasan, Jean Gautier
    Pages 119-158
  5. Tovah A. Day, Chris Sproul, Marila Cordeiro-Stone, Cyrus Vaziri
    Pages 159-170
  6. Claudia Tapia-Alveal, Matthew J. O’Connell
    Pages 171-179
  7. Lois Resnick-Silverman, James J. Manfredi
    Pages 193-203
  8. Michael DeRan, Mary Pulvino, Jiyong Zhao
    Pages 221-230

About this book

Introduction

Cell cycle checkpoints control the fidelity and orderly progression of eukaryotic cell division. By controlling the orderly progression of critical cell cycle events such as DNA replication and chromosome segregation and ensuring proper repair of damaged DNA, cell cycle checkpoints function to ensure genome integrity. Mechanisms of checkpoint controls are not only the research focus of investigators interested in mechanisms that regulate the cell cycle, but are also the interests of researchers studying cancer development as it is increasingly clear that loss of cell cycle checkpoints, which leads to genomic instability as a result, is a hallmark of tumorigenesis. Cell Cycle Checkpoints: Methods and Protocols provides detailed descriptions of methodologies currently employed by researchers in the field, including those commonly used in the mammalian, yeast, C. elegans, Drosophila, and Xenopus model systems. Each chapter describes a specific technique or protocol, such as a method to induce cell cycle checkpoints in a particular model system, to synchronize a population of cells to allow observations of cell cycle progression, to identify genes involved in checkpoint regulation, and to study particular protein components of cell cycle checkpoint pathways. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Cell Cycle Checkpoints: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential field.

Keywords

DNA damage checkpoints apoptosis cell cycle progression checkpoint kinases eukaryotic cell division fission yeast schizosaccharomyces pombe spindle assembly checkpoint

Editors and affiliations

  • Willis X. Li
    • 1
  1. 1., Department of MedicineUniversity of CaliforniaLa JollaUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-273-1
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-272-4
  • Online ISBN 978-1-61779-273-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book