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Proteomic Analysis of Laser Microdissected Ovarian Cancer Tissue with SELDI-TOF MS

  • Isabelle CadronEmail author
  • Toon Van Gorp
  • Philippe Moerman
  • Etienne Waelkens
  • Ignace Vergote
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 755)

Abstract

The evolution of ovarian cancer will highly depend on platinum sensitivity or resistance. In an attempt to better understand this mechanism of resistance, we combined laser microdissection (LMD) of ovarian tumor cells with mass spectrometric techniques. To obtain disease-specific markers we isolated ovarian tumor cells with LMD without contamination of surrounding cells. Proteins were extracted by chemical lysis and subsequent peptide and protein information was gathered using surface-enhanced laser desorption/ionization-time of flight mass spectrometry.

Key words

Ovarian cancer SELDI-TOF MS Platinum resistance Laser microdissection Biomarkers 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Isabelle Cadron
    • 1
    Email author
  • Toon Van Gorp
    • 1
    • 2
  • Philippe Moerman
    • 3
  • Etienne Waelkens
    • 4
  • Ignace Vergote
    • 1
  1. 1.Division of Gynecological Oncology, Department of Obstetrics and GynecologyUniversity Hospitals Leuven, Katholieke Universiteit LeuvenLeuvenBelgium
  2. 2.Division of Gynaecological Oncology, Department of Obstetrics and GynaecologyMUMC+, GROW – School for Oncology and Developmental BiologyMaastrichtThe Netherlands
  3. 3.Department of PathologyUniversity Hospitals Leuven, Katholieke Universiteit LeuvenLeuvenBelgium
  4. 4.Department of Molecular Cell BiologyUniversity Hospitals Leuven, Katholieke Universiteit LeuvenLeuvenBelgium

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