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Imaging Fluorescently Tagged Phytophthora Effector Proteins Inside Infected Plant Tissue

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Plant Immunity

Part of the book series: Methods in Molecular Biology ((MIMB,volume 712))

Abstract

Assays to determine the role of pathogen effectors within an infected plant cell are yielding valuable information about which host processes are targeted to allow successful pathogen colonization. However, this does not necessarily inform on the cellular location of these interactions, or if these effector–virulence target interactions occur only in the presence of the pathogen. Here, we describe techniques to allow the subcellular localization of pathogen effectors inside infected plant cells or tissues, based largely on infiltration of plant tissue by Agrobacterium tumefaciens and its delivery of DNA encoding fluorescent protein-tagged effectors, and subsequent confocal microscopy.

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Acknowledgments

PCB and SW are funded by the Scottish Government Rural and Environment Research and Analysis Directorate (RERAD) and the Biotechnology and Biological Sciences Research Council (BBSRC), UK. PRJB is funded by the University of Dundee, UK, and the BBSRC.

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Authors and Affiliations

  1. Plant Pathology Programme, Scottish Crop Research Institute, Invergowrie, Dundee, UK

    Petra C. Boevink & Stephen C. Whisson

  2. Division of Plant Sciences, College of Life Sciences, University of Dundee at SCRI, Invergowrie, Dundee, UK

    Paul R. J. Birch

Authors
  1. Petra C. Boevink
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  2. Paul R. J. Birch
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  3. Stephen C. Whisson
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Corresponding author

Correspondence to Stephen C. Whisson .

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Editors and Affiliations

  1. , Plant Pathology, Physiol., & Weed Sci., Virginia Tech, Latham Hall (0390) 550, Blacksburg, 24061, Virginia, USA

    John M. McDowell

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Boevink, P.C., Birch, P.R.J., Whisson, S.C. (2011). Imaging Fluorescently Tagged Phytophthora Effector Proteins Inside Infected Plant Tissue. In: McDowell, J. (eds) Plant Immunity. Methods in Molecular Biology, vol 712. Humana Press. https://doi.org/10.1007/978-1-61737-998-7_16

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  • DOI: https://doi.org/10.1007/978-1-61737-998-7_16

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61737-997-0

  • Online ISBN: 978-1-61737-998-7

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