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Instruments and Methods in Proteomics

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Data Mining in Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 696))

Abstract

In the past decade, major developments in instrumentation and methodology have been achieved in proteomics. For proteome investigations of complex biological samples derived from cell cultures, tissues, or whole organisms, several techniques are state of the art. Especially, many improvements have been undertaken to quantify differences in protein expression between samples from, e.g., treated vs. untreated cells and healthy vs. control patients. In this review, we give a brief insight into the main techniques, including gel-based protein separation techniques, and the growing field of mass spectrometry.

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Acknowledgments

FB, PC, CS, BS, and KM are funded by the BMBF (grant 01 GS 08143). CM is supported by the Alma-Vogelsang Foundation.

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  1. Department of Functional Proteomics, Medizinisches Proteom-Center, Ruhr-Universität Bochum, Bochum, Germany

    Caroline May, Frederic Brosseron, Piotr Chartowski, Cornelia Schumbrutzki, Bodo Schoenebeck & Katrin Marcus

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  1. Caroline May
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  2. Frederic Brosseron
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Correspondence to Bodo Schoenebeck .

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  1. Lead Discovery Center GmbH, Emil-Figge-Straße 76a76a, Dortmund, 44227, Germany

    Michael Hamacher

  2. Medizinisches Proteom-Center, Ruhr-Universität Bochum, Universitätsstraße 150, Bochum, 44801, Germany

    Martin Eisenacher

  3. Medizinisches Proteom-Center, Ruhr-Universität Bochum, Universitätsstraße 150, Bochum, 44801, Germany

    Christian Stephan

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© 2011 Springer Science+Business Media, LLC

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May, C., Brosseron, F., Chartowski, P., Schumbrutzki, C., Schoenebeck, B., Marcus, K. (2011). Instruments and Methods in Proteomics. In: Hamacher, M., Eisenacher, M., Stephan, C. (eds) Data Mining in Proteomics. Methods in Molecular Biology, vol 696. Humana Press. https://doi.org/10.1007/978-1-60761-987-1_1

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  • DOI: https://doi.org/10.1007/978-1-60761-987-1_1

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-986-4

  • Online ISBN: 978-1-60761-987-1

  • eBook Packages: Springer Protocols

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