Abstract
As DNA methylation analysis enters the mainstream of biomedical research, there is increasing interest in methodologies that can be used for rapid analysis of a wide variety of biological and clinical samples. The methods that have been commonly used for methylation analysis are usually time-consuming and require multiple steps. The methylation-sensitive high-resolution melting (MS-HRM) assay is an in-tube assay which was developed to assess promoter methylation in sodium bisulphite-modified DNA and is particularly useful for assessing methylation in short fragments of DNA derived from formalin-fixed paraffin-embedded biopsies.
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Acknowledgements
EMW was supported by the Dora Lush Biomedical Postgraduate Scholarship from the National Health and Medical Research Council of Australia. Development and continued studies of MS-HRM were made possible by grants to AD from the National Health and Medical Research Council of Australia, the US Department of Defense Breast Cancer Research Program under award number W81XWH-06-1-0670, the Cancer Council of Victoria and the National Breast Cancer Foundation of Australia. Views and opinions of, and endorsements by the authors do not reflect those of the US Army or the Department of Defense.
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© 2011 Humana Press
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Wong, E.M., Dobrovic, A. (2011). Assessing Gene-Specific Methylation Using HRM-Based Analysis. In: Park, D. (eds) PCR Protocols. Methods in Molecular Biology, vol 687. Humana Press. https://doi.org/10.1007/978-1-60761-944-4_14
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DOI: https://doi.org/10.1007/978-1-60761-944-4_14
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