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Measurement of Mitochondrial ROS Production

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Protein Misfolding and Cellular Stress in Disease and Aging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 648))

Abstract

The significance of reactive oxygen species (ROS) as aggravating or primary factors in numerous pathologies is widely recognized, with mitochondria being considered the major intracellular source of ROS. It is not yet possible to routinely measure mitochondrial ROS in animals or cultured cells with a reasonable degree of certainty. However, at the level of isolated mitochondria, one can easily monitor and quantify the rate of ROS production, identify major sites of ROS production, and compare the rates of ROS production in mitochondria isolated from normal and diseased tissue. In this chapter, we describe in detail the most recent and reliable method to measure mitochondrial ROS as the rate of H2O2 emission. This method may be employed with minimal modifications to measure H2O2 production by mitochondria isolated from various tissues and under a wide variety of experimental conditions.

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Acknowledgement

This work was supported by NIH/NIA grant AG014930.

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Authors and Affiliations

  1. Department of Neurology and Neuroscience, Weill Medical College of Cornell University, New York, NY, USA

    Anatoly A. Starkov

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  1. Anatoly A. Starkov
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Correspondence to Anatoly A. Starkov .

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Editors and Affiliations

  1. Aarhus University Hospital, Research Unit for Molecular Medicine, University of Aarhus, Brendstrupgaardsvej 100, Aarhus N, 8200, Denmark

    Peter Bross

  2. Aarhus University Hospital, Research Unit for Molecular Medicine, University of Aarhus, Brendstrupgaardsvej 100, Aarhus N, 8200, Denmark

    Niels Gregersen

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Starkov, A.A. (2010). Measurement of Mitochondrial ROS Production. In: Bross, P., Gregersen, N. (eds) Protein Misfolding and Cellular Stress in Disease and Aging. Methods in Molecular Biology, vol 648. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-756-3_16

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  • DOI: https://doi.org/10.1007/978-1-60761-756-3_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-755-6

  • Online ISBN: 978-1-60761-756-3

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