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Quantitation of Fentanyl in Blood and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS)

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Clinical Applications of Mass Spectrometry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 603))

Abstract

Fentanyl is a potent, short-acting synthetic opioid analgesic. Fentanyl is measured in blood and urine following mixed-mode solid phase extraction. The specimens are fortified with deuterated internal standard and a five-point calibration curve is constructed. The final extracts are reconstituted in methanol and analyzed using selected ion monitoring gas chromatography-mass spectrometry.

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© 2010 Humana Press, a part of Springer Science+Business Media, LLC

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Goldberger, B.A., Chronister, C.W., Merves, M.L. (2010). Quantitation of Fentanyl in Blood and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS). In: Garg, U., Hammett-Stabler, C. (eds) Clinical Applications of Mass Spectrometry. Methods in Molecular Biology, vol 603. Humana Press. https://doi.org/10.1007/978-1-60761-459-3_22

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  • DOI: https://doi.org/10.1007/978-1-60761-459-3_22

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-458-6

  • Online ISBN: 978-1-60761-459-3

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