Abstract
Three-dimensional (3D) organotypic models are increasingly being used to study aspects of epidermal organisation and cutaneous wound-healing events. These are largely dependent on laborious histological analysis and immunohistochemical approaches. Here we outline a method for establishment of a versatile in vitro 3D organotypic skin equivalent that reflects murine epidermal organisation in vivo. The system is optically transparent and ideally suited to real-time analysis and integrated in situ imaging techniques. Moreover, the model permits the visualisation of epidermal regeneration following injury in real time, thereby facilitating avenues to explore distinctive modes of wound re-epithelialisation. The versatility of the model could help unravel molecular mechanisms underlying epidermal morphogenesis, assess novel therapeutic strategies and reduce animal experimentation in a non-invasive manner.
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Acknowledgements
The authors would like to thank Glasgow Caledonian University Bioscience Lab for their assistance in providing mouse samples for the model and Dr. Irwin Mclean for kindly gifting the polyclonal filaggrin antibody. This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC), grant no. BB/C005058.
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Kandyba, E., Hodgins, M., Martin, P. (2010). A Versatile Murine 3D Organotypic Model to Evaluate Aspects of Wound Healing and Epidermal Organization. In: Turksen, K. (eds) Epidermal Cells. Methods in Molecular Biology, vol 585. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-380-0_21
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DOI: https://doi.org/10.1007/978-1-60761-380-0_21
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60761-379-4
Online ISBN: 978-1-60761-380-0
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