Epidermal Cells

Methods and Protocols

  • Kursad Turksen

Part of the Methods in Molecular Biology book series (MIMB, volume 585)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Corina Lorz, Carmen Segrelles, Marina Garín, Jesús M. Paramio
    Pages 1-11
  3. Federica Riva, Andrea Casasco, Marco Casasco, Alberto Calligaro, Antonia Icaro Cornaglia
    Pages 25-43
  4. Frédéric Minner, Françoise Herphelin, Yves Poumay
    Pages 71-82
  5. Christian M. Metallo, Lin Ji, Juan J. de Pablo, Sean P. Palecek
    Pages 83-92
  6. Lina Dagnino, Ernest Ho, Wing Y. Chang
    Pages 93-105
  7. Cory L. Simpson, Shin-ichiro Kojima, Spiro Getsios
    Pages 127-146
  8. Igor Gurevich, Carmen Zhang, Brian J. Aneskievich
    Pages 147-158
  9. Ramón García-Escudero, Jesús M. Paramio
    Pages 171-181
  10. Martin Ruetze, Katrin Lochner, Stefan Gallinat, Anja Knott
    Pages 183-192
  11. Jianjun Shen, Susan M. Fischer
    Pages 225-252
  12. Heena Panchal, Olivia Wansbury, Beatrice A. Howard
    Pages 261-270
  13. Carolyn Byrne, Ariel A. Avilion, Ryan F. O’Shaughnessy, Jonathan C. Welti, Matthew J. Hardman
    Pages 271-286

About this book

Introduction

In recent years, our ability to understand and manipulate epidermal cells has increased tremendously, opening significant new possibilities in both basic science research and in regenerative medicine, including would healing and transplantation. Epidermal Cells: Methods and Protocols, Second Edition expands upon the popular first edition by bringing together a panel of experienced basic and clinical researchers to describe in step-by-step detail the powerful methods they have developed and optimized to analyze and manipulate epidermal cell precursors and mature epidermal cells. These protocols cover different methods and models for culturing epidermal cells, for enriching very early epidermal progenitors, as well as for studying epidermal cell commitment and differentiation both in vitro and in vivo. Topics of special interest include the derivation, characterization, and utility of epidermal stem cells, mature epidermal cells and their characterization, and applications in regenerative medicine. The protocols follow the successful Methods in Molecular Biology™ series format, providing introductions to their respective topics, lists of the necessary materials and reagents, and notes on troubleshooting and avoiding known pitfalls.

State-of-the-art and highly practical, Epidermal Cells: Methods and Protocols, Second Edition offers experienced and novice investigators alike an invaluable collection of readily reproducible techniques designed to broaden not only our understanding of the biology of epidermal cells, but also their utility in normal tissue homeostasis and regenerative medicine applications.

Keywords

DNA Endoplasmatisches Reticulum Fische Microarray Precursors Progenitors Proteomics Vivo gene expression genes hybridization molecular biology reproducible techniques tissue transcription

Editors and affiliations

  • Kursad Turksen
    • 1
  1. 1.Sprott Centre for Stem Cell ResearchOttawa Hosp. Research Institute (OHRI)OttawaCanada

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60761-380-0
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-379-4
  • Online ISBN 978-1-60761-380-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book