Abstract
Gels can be fragile and difficult to handle and store without suffering damage. This can be overcome by drying, whereby they become bonded to a stabilizing medium. Furthermore, it may be necessary to dry a gel in order to obtain the most efficient detection of radioactive samples on it by autoradiography, and fluorography. In this chapter, two methods are described—the first being drying onto absorbent paper (this being suitable for storage, reflection densitometry, and autoradiography), and the second being drying between cellulose sheets (this being suitable for storage, transmission densitometry, and demonstration by overhead projection). Both methods are suitable for slab gels—tube-shaped gels are not suited to drying down.
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References
Joshi, S. and Haenni, A. L. (1980) Fluorographic detection of nucleic acids labeled with weak β-emitters in gels containing high acrylamide concentrations. FEES Lett. 118, 43–46.
Bio-Rad Inc. (1981) Model 1125B high capacity gel slab dryer for protein gels and DNA sequencing. Bio-Rad Bulletin 1079, Bio-Rad Inc., Hercules, CA.
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© 1996 Humana Press Inc., Totowa, NJ
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Smith, B.J. (1996). Drying Polyacrylamide Gels. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-259-9_34
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DOI: https://doi.org/10.1007/978-1-60327-259-9_34
Publisher Name: Humana Press
Print ISBN: 978-0-89603-338-2
Online ISBN: 978-1-60327-259-9
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