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The Protein Protocols Handbook

  • John M. Walker

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Quantitation of Proteins

    1. Front Matter
      Pages 1-1
    2. Alastair Aitken, Michèle Learmonth
      Pages 3-6
    3. Jakob H. Waterborg, Harry R. Matthews
      Pages 7-9
    4. Nicholas J. Kruger
      Pages 15-20
    5. Robert E. Akins, Rocky S. Tuan
      Pages 21-28
    6. Alastair Aitken, Michèle Learmonth
      Pages 29-31
    7. F. Andrew Ray, Thomas D. Friedrich, Judith Laffin, John M. Lehman
      Pages 33-38
    8. Douglas D. Root, Kuan Wang
      Pages 39-43
  3. Electrophoresis of Proteins and Peptides and Detection in Gels

    1. Front Matter
      Pages 49-49
    2. Jakob H. Waterborg
      Pages 91-100
    3. Christopher F. Thurston, Lucy F. Henley
      Pages 115-119
    4. Graeme R. Guy, Robin J. Philp
      Pages 133-137
    5. David J. Begley
      Pages 143-154
    6. Joan-Ramon Daban, Salvador Bartolomé, Antonio Bermúdez
      Pages 179-185
    7. Juan M. García-Segura, Mercedes Ferreras
      Pages 187-195
    8. Jung-Kap Choi, Hee-Youn Hong, Gyurng-Soo Yoo
      Pages 197-201
    9. Paul Jenö, Martin Horst
      Pages 207-214
    10. Serge Desnoyers, Sylvie Bourassa, Guy G. Poirier
      Pages 215-222
    11. Bryan John Smith
      Pages 223-227
    12. Antonella Circolo, Sunita Gulati
      Pages 235-242
  4. Blotting and Detection Methods

    1. Front Matter
      Pages 243-243
    2. Mark Page, Robin Thorpe
      Pages 245-247
    3. Patricia Gravel, Olivier Golaz
      Pages 249-260
    4. John M. Walker
      Pages 261-262
    5. G. Brian Wisdom
      Pages 271-272
    6. Rosaria P. Haugland, Wendy W. You
      Pages 293-301

About this book

Introduction

In The Protein Protocols Handbook, I have attempted to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benehtop manual and guide both for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. We each, of course, have our own favorite, commonly used gel system, g- staining method, blotting method, and so on; I'm sure you will find yours here. H- ever, I have also described a variety of altematives for many of these techniques; though they may not be superior to the methods you commonly use, they may nev- theless be more appropriate in a particular situation. Only by knowing the range of techniques that are available to you, and the strengths and limitations of these te- niques, will you be able to choose the method that best suits your purpose.

Keywords

chemistry protein proteins

Editors and affiliations

  • John M. Walker
    • 1
  1. 1.University of HertfordshireHatfieldUK

Bibliographic information