Abstract
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) has proven to be among the most useful tools yet developed in the area of molecular biology. The discontinuous buffer system, first described by Laemmli (1), has made it possible to separate, visualize, and compare readily the component parts of complex mixtures of molecules (e.g., tissues, cells). SDS-PAGE separation of proteins and peptides makes it possible to quantify the amount of a particular protein/peptide in a sample, obtain fairly reliable molecular mass information, and, by combining SDS-PAGE with immunoelectroblotting, evaluate the antigenicity of proteins and peptides. SDS-PAGE is both a powerful separation system and a reliable preparative purification technique (2 and see Chapter 11).
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References
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© 1996 Humana Press Inc., Totowa, NJ
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Judd, R.C. (1996). SDS-Polyacrylamide Gel Electrophoresis of Peptides. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-259-9_16
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DOI: https://doi.org/10.1007/978-1-60327-259-9_16
Publisher Name: Humana Press
Print ISBN: 978-0-89603-338-2
Online ISBN: 978-1-60327-259-9
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