Demyelination is a significant component of contusive spinal cord injury as well as in multiple sclerosis. As such, remyelination, either by endogenous or grafted exogenous myelinating cells is a viable therapeutic target to restore function. To assess specific approaches to facilitate functional remyelination in vivo, appropriate injury models are needed. This chapter will discuss the strengths and weaknesses of a number of demyelinating lesions of the spinal cord and provide guidelines for choosing which model best suits which experimental condition. Step by step procedures for both creating and assessing the lesion will be provided.
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References
Hall SM, Gregson NA. The in vivo and ultrastructural effects of injection of lyso-phosphatidyl choline into myelinated peripheral nerve fibres of the adult mouse. J Cell Sci 1971; 9:769–789.
Dubois-Dalcq ME, Doller EW, Haspel MV, Holmes KV. Cell tropism and expression of mouse hepatitis viruses (MHV) in mouse spinal cord cultures. Virology 1982; 119:317–331.
Matsushima GK, Morell P. The neurotoxicant, cuprizone, as a model to study demyelination and remyelination in the central nervous system. Brain Pathol 2001; 11:107–116.
Keirstead HS, Blakemore WF. Identification of post-mitotic oligodendrocytes incapable of remyelination within the demyelinated adult spinal cord. J Neuropathol Exp Neurol 1997; 56:1191–1201.
Woodruff RH, Franklin RJ. Demyelination and remyelination of the caudal cerebellar peduncle of adult rats following stereotaxic injections of lysolecithin, ethidium bromide, and complement/anti-galactocerebroside: a comparative study. Glia 1999; 25:216–228.
Loy DN, Magnuson DSK, Zhang YP et al. Functional redundancy of ventral spinal locomotor pathways. J Neurosci 2002; 22:315–323.
Zhang YP, Shields LB, Zhang Y et al. Use of magnetic stimulation to elicit motor evoked potentials, somatosensory evoked potentials, and H-reflexes in non-sedated rodents. J Neurosci Methods 2007; 165:9–17.
Zhang YP, Burke DA, Shields LBE et al. Combined assessment to discriminate small functional differences following spinal cord contusion based on precise vertebral stabilization and tissue displacement. J Neurotrauma (submitted).
Cao QL, Zhang Y P, Iannotti C et al. Functional and electrophysiological consequences of graded contusive spinal cord injury in the adult rat. Exp Neurol 2005; 191:S3–S16.
Beaumont E, Onifer SM, Reed WR, Magnuson DSK. Magnetically-evoked inter-enlargement response: An assessment of ascending propriospinal fibers following spinal cord injury. Exp Neurol (in press).
Totoiu MO, Keirstead HS. Spinal cord injury is accompanied by chronic progressive demyelination. J Comp Neurol 2005; 486:373–383.
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© 2009 Humana Press, a part of Springer Science + Business Media, LLC
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Jernigan, S.C., Zhang, Y.P., Shields, C.B., Whittemore, S.R. (2009). Rodent Spinal Cord Demyelination Models. In: Chen, J., Xu, Z.C., Xu, XM., Zhang, J.H. (eds) Animal Models of Acute Neurological Injuries. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-185-1_40
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DOI: https://doi.org/10.1007/978-1-60327-185-1_40
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