Abstract
Numerous shoots were directly regenerated from the leaf explants of Lysionotus pauciflorus on the MS medium containing 0.5–2 µM NAA with or without 1 µM BA. The calli were induced from the leaves on MS medium supplemented with 2 µM 2, 4-D. The calli proliferated about four times in fresh weight in the liquid medium of the same composition as the callus induction medium after 4 weeks of culture on a rotary shaker at 100 rpm. Shoots were induced from these calli on the regeneration medium amended with 32 µM BA or 0.5 µM zeatin. Regenerated shoots rooted easily on ½ MS medium without any plant growth regulators. Most of the regenerants from callus were diploid, whereas eight of 66 acclimatized plantlets were tetraploid determined by flow cytometric analysis. Furthermore, flow cytometric analysis of calli also revealed tetraploidy.
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Acknowledgments
We wish to thank Dr. Masashi Nakata of Botanic Gardens of Toyama for chromosome observations, Dr. Tohru. Ohmiya of Botanic Gardens of Toyama for cytological observation, Dr. Dong Poh Chin of Chiba University for flow cytometric analysis and Dr. Kaiyun Guan of Kunming Institute of Botany for useful information.
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Godo, T., Lu, Y., Mii, M. (2010). Micropropagation of Lysionotus pauciflorus Maxim. (Gesneriaceae). In: Jain, S., Ochatt, S. (eds) Protocols for In Vitro Propagation of Ornamental Plants. Methods in Molecular Biology, vol 589. Humana Press. https://doi.org/10.1007/978-1-60327-114-1_13
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DOI: https://doi.org/10.1007/978-1-60327-114-1_13
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