Summary
Daylily plantlets with tetraploid or octaploid chromosome numbers were induced from colchicine-treated diploid callus (2n=22). Callus tissue of the daylily Hemerocallis flava L. was placed on a morphogenetic induction medium, a modified MS formula supplemented with 1 mg 2,4-D and 1 mg kinetin/liter, plus 0,10, 20, or 40 mg/liter colchicine in the dark at 12°C for 3 days and recuperated for 1 week under the same environmental conditions except that colchicine was left out. The calluses were then returned to the normal growth temperature 25°C. Ploidy was identified by chromosome counts of squashed root-tip cells collected from the newly potted plantlets and later by measurements of stomates and pollen grains. Over 50% of the plants initiated from the colchicine-treated calluses were completely tetraploid. All of the plantlets differentiated from untreated callus had a diploid number of 22. Of the 3 colchicine levels applied, the 20 mg/liter treatment appeared to be the most effective in production of tetraploidy.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arisumi, T., 1964. Colchicine-induced tetraploid and cytochimeral daylilies. J. Heredity 55: 254–260.
Arisumi, T., 1972. Stabilities of colchicine-induced tetraploid and cytochimeral daylilies. J. Heredity 63: 15–18.
Bayliss, M. W., 1973. Origin of chromosome number variation in cultured plant cells. Nature 246: 529–530.
Chen, C. H. & D. J. Holden 1972. Organogenesis in daylily callus. Proc. S. D. Acad. Sci. 51: 146–149.
Chen, C. H., P. F. Lo & J. G. Ross, 1979. Regeneration of plantlets from callus cultures of Indiangrass. Crop Sci. 19: 117–118.
Chen, C. H., N. E. Stenberg & J. G. Ross, 1977. Clonal propagation of big bluestem by tissue culture. Crop Sci. 17: 847–850.
Goeden-Kallemeyn, Y. C. & C. H. Chen, 1978. Consistency of the chromosome constitution in plants derived from a long-term daylily callus culture. Proc. S. D. Acad. Sci. 57: 108–112.
Griesbach, R. A., O. W. Fay & L. Horsfall, 1963. Induction of polyploidy in newly-germinated Hemerocallis seedlings. Hemerocallis J. 17: 70–75.
Heinz, D. J. & D. W. P. Mee, 1971. Morphologic, cytogenetic and enzymatic variation in Saccharum species hybrid clones derived from callus tissue. Am. J. Bot. 58: 257–262.
Heuser, C. W. & D. A. Apps, 1976. In vitro plantlet formation from flower petal explats of Hemerocallis cv ‘Chipper Cherry’. Can. J. Bot. 54: 616–618.
Horak, J. J. Lustinee, J. Mesicek, & D. Polackiva, 1975. Regeneration of diploid and polyploid plants from the stem pith explants of diploid marrow stem kale. Ann. Bot 39: 571–577.
Meyer, M. M., 1976. Propagation of daylilies by tissue culture. HortScience 11: 485–487.
Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco culture. Plant Physiol. 15: 473–497.
Peck, V. L. & T. Arisumi, 1968. Tetraploid daylilies. Am. Hort. Mag. 47: 169–171.
Saunders, J. W. & E. T. Bingham, 1972. Production of alfalfa plants from callus tissue. Crop Sci. 12: 804–808.
Stenberg, N. E., C. H. Chen & J. G. Ross, 1977. Regeneration of plantslets from leaf cultures of Lilium longiflorum Thumb. Proc. S. D. Acad. Sci. 56: 152–158.
Traub, H. P., 1951. Colchicine-induced Hemerocallis polyploids and their breeding behavior. Plant Life 7: 83–116.
Williams, L. & H. A. Collin, 1976. Growth and cytology of celery plants derived from tissue cultures. Ann. Bot. 40: 333–338.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chen, C.H., Goeden-Kallemeyn, Y.C. In vitro induction of tetraploid plants from colchicine-treated diploid daylily callus. Euphytica 28, 705–709 (1979). https://doi.org/10.1007/BF00038937
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00038937