Summary
Ultrasound-based approach for enhanced protein blotting is proposed. Three minutes of ultrasound exposure (1 MHz, 2.5 W/cm2) was sufficient for a clear transfer of proteins from a polyacrylamide gel (PhastGel) to nitrocellulose or Nylon 66 Biotrans membrane. The proteins evaluated were prestained sodium dodecyl sulfate-polyacrylamide standards (18,500–106,000 Da) and 14C-labeled Rainbow protein molecular weight markers (14,300–200,000 Da).
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Kost, J. (2009). Blotting from PhastGel to Membranes by Ultrasound. In: Kurien, B., Scofield, R. (eds) Protein Blotting and Detection. Methods in Molecular Biology, vol 536. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-542-8_19
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DOI: https://doi.org/10.1007/978-1-59745-542-8_19
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