Protein Blotting and Detection

Methods and Protocols

  • Biji T. Kurien
  • R. Hal Scofield

Part of the Methods in Molecular Biology book series (MIMB, volume 536)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Harry Towbin
    Pages 1-3
  3. Biji T. Kurien, R. Hal Scofield
    Pages 9-22
  4. Graeme Lowe
    Pages 67-73
  5. Rafael F. Pont-Lezica
    Pages 75-88
  6. S. Sumi, A. Mathai, V.V. Radhakrishnan
    Pages 89-93
  7. Dalit Talmi-Frank, Charles L. Jaffe, Gad Baneth
    Pages 103-113
  8. Engelbert Buxbaum
    Pages 115-128
  9. Ulrich Canzler, Holger Bartsch, Kai Großmann, Werner Lehmann, Karsten Conrad, Biji T. Kurien et al.
    Pages 129-137
  10. Joon-Yong Chung, Stephen M. Hewitt
    Pages 139-148
  11. Anatoly Kiyatkin, Edita Aksamitiene
    Pages 149-161

About this book


Over the past thirty years, the development of the Western blot has revolutionized the fields of biomedical research and medical diagnostics. In Protein Blotting and Detection: Methods and Protocols, expert researchers present numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting. Chapters offer a large number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional techniques as well as completely original methods of protein blotting. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, a list of necessary materials, step-by-step methods, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Innovative and highly practical, Protein Blotting and Detection: Methods and Protocols is an essential, hands-on guide for all investigators who hope to bring these cutting-edge procedures home to their laboratories.


Immunoblotting Membrane proteomics Polyacrylamid-Gelelektrophorese Protein transfer Proteomics SDS-PAGE TLC-Blot Western blotting molecular biology

Editors and affiliations

  • Biji T. Kurien
    • 1
  • R. Hal Scofield
    • 2
  1. 1.Oklahoma Medical Research FoundationOklahoma CityU.S.A.
  2. 2.Oklahoma Medical Research FoundationOklahoma CityU.S.A.

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-934115-73-2
  • Online ISBN 978-1-59745-542-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book