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Localizing Proteins by Tissue Printing

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Protein Blotting and Detection

Part of the book series: Methods in Molecular Biology ((MIMB,volume 536))

Summary

The simple technique of making tissue prints on appropriate substrate material has made possible the easy localization of proteins, nucleic acids, carbohydrates, and small molecules in a tissue-specific mode. Plant tissues can be used to produce prints revealing a remarkable amount of anatomical detail, even without staining, which might be used to record developmental changes over time. In this chapter we will focus on the protocols for the localization of proteins and glycans using antibodies or lectins, probably the most frequently used application, but the localization of other molecules is reported and the sources indicated.

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Acknowledgements

The author is grateful to the Université Paul Sabatier (Toulouse III, France) and the CNRS for support.

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Authors and Affiliations

  1. Surfaces Cellulaires et Signalisation chez les Végétaux, UMR 5546 CNRS-Université Paul Sabatier-Toulouse III, Pôle de Biotechnologie végétale, 24 Chemin de Borde Rouge BP, 42617 Auzeville, 31326, Castanet-Tolosan, France

    Rafael F. Pont-Lezica

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  1. Rafael F. Pont-Lezica
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Correspondence to Rafael F. Pont-Lezica .

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Editors and Affiliations

  1. Oklahoma Medical Research Foundation, NE. 13th Street 825, Oklahoma City, 73104, U.S.A.

    Biji T. Kurien

  2. Oklahoma Medical Research Foundation, NE. 13th Street 825, Oklahoma City, 73104, U.S.A.

    R. Hal Scofield

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Pont-Lezica, R.F. (2009). Localizing Proteins by Tissue Printing. In: Kurien, B., Scofield, R. (eds) Protein Blotting and Detection. Methods in Molecular Biology, vol 536. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-542-8_10

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  • DOI: https://doi.org/10.1007/978-1-59745-542-8_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-934115-73-2

  • Online ISBN: 978-1-59745-542-8

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