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Mass Spectrometric Characterization of Neuropeptides

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Mass Spectrometry of Proteins and Peptides

Part of the book series: Methods In Molecular Biology ((MIMB,volume 492))

Summar

Because of their great biological significance, neuropeptides are the subject of intensive research. Mass spectrometry (MS) is a highly informative and sensitive method used for detecting and characterizing these compounds. Successful MS analysis of neuropeptides is dependent on careful sample preparation. Herein, we present two common sample preparation strategies: direct tissue analysis and pooled tissue extraction coupled with fractionation.

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Acknowledgments

This work was supported in part by National Science Foundation CAREER Award (CHE-0449991), National Institutes of Health through grant 1R01DK071801, and an Alfred P. Sloan Research Fellowship (L.L.). S.S.C. is supported by an individual NRSA predoctoral fellowship 1F31NS053283. J.A.D. acknowledges an American Foundation for Pharmaceutical Education (AFPE) pre-doctoral fellowship.

Author information

Authors and Affiliations

  1. Department of Chemistry, School of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA

    James A. Dowell & Lingjun Li

Authors
  1. Stephanie S. Cape
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  2. James A. Dowell
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  3. Lingjun Li
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Editor information

Editors and Affiliations

  1. Fundamental & Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, USA

    Mary S. Lipton

  2. Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA

    Ljiljana Paša-Tolic

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Cape, S.S., Dowell, J.A., Li, L. (2009). Mass Spectrometric Characterization of Neuropeptides. In: Lipton, M.S., Paša-Tolic, L. (eds) Mass Spectrometry of Proteins and Peptides. Methods In Molecular Biology, vol 492. Humana Press. https://doi.org/10.1007/978-1-59745-493-3_23

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  • DOI: https://doi.org/10.1007/978-1-59745-493-3_23

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-934115-48-0

  • Online ISBN: 978-1-59745-493-3

  • eBook Packages: Springer Protocols

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