Skip to main content
SpringerLink
Log in
Book cover

Mass Spectrometry of Proteins and Peptides

Methods and Protocols, Second Edition

  • Book
  • © 2009

Overview

Editors:
  1. Mary S. Lipton

    1. Fundamental & Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Ljiljana Paša-Tolic

    1. Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Comprehensive overview of latest mass spectrometric techniques for protein analysis
  • Step-by-step methods complete with detailed instructions for successful implementation
  • Written by established experts with hands-on experience
  • Extensive, up-to-date references arranged by application
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 492)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (28 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Generation and Analysis of Multidimensional Protein Identification Technology Datasets

    • Selene K. Swanson, Laurence Florens, Michael P. Washburn
    Pages 1-20

  3. Quantitative Peptide and Protein Profiling by Mass Spectrometry

    • Alexander Schmidt, Birgit Bisle, Thomas Kislinger
    Pages 21-38

  4. Label-Free Relative Quantitation of Prokaryotic Proteomes Using the Accurate Mass and Time Tag Approach

    • Kim K. Hixson
    Pages 39-63

  5. Classical Proteomics: Two-Dimensional Electrophoresis/MALDI Mass Spectrometry

    • Ursula Zimny-Arndt, Monika Schmid, Renate Ackermann, Peter R. Jungblut
    Pages 65-91

  6. The Use of Difference In-Gel Electrophoresis for Quantitation of Protein Expression

    • Rajat Sapra
    Pages 93-112

  7. Liquid-Chromatography-Mass Spectrometry of Thylakoid Membrane Proteins

    • Christian G. Huber, Anna-Maria Timperio, Hansjörg Toll, Lello Zolla
    Pages 113-130

  8. High Accuracy Mass Spectrometry in Large-Scale Analysis of Protein Phosphorylation

    • Jesper V. Olsen, Boris Macek
    Pages 131-142

  9. Manual Validation of Peptide Sequence and Sites of Tyrosine Phosphorylation from MS/MS Spectra

    • Amy M. Nichols, Forest M. White
    Pages 143-160

  10. Assigning Glycosylation Sites and Microheterogeneities in Glycoproteins by Liquid Chromatography/Tandem Mass Spectrometry

    • Yehia Mechref, Milan Madera, Milos V. Novotny
    Pages 161-180

  11. Structure Analysis of N -Glycoproteins

    • Stefanie Henning, Jasna Peter-Katalinić, Gottfried Pohlentz
    Pages 181-200

  12. Capillary Zone Electrophoresis-Mass Spectrometry for the Characterization of Isoforms of Intact Glycoproteins

    • Christian Neusüβ, Matthias Pelzing
    Pages 201-213

  13. Top-Down Proteomics on a High-field Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

    • Séverine A. Ouvry-Patat, Matthew P. Torres, Craig A. Gelfand, Hung Hiang Quek, Michael Easterling, J. Paul Speir et al.
    Pages 215-231

  14. Capillary Isoelectric Focusing/Reversed Phase Liquid Chromatography/Mass Spectrometry

    • Cheng S. Lee, Brian M. Balgley
    Pages 233-240

  15. Integrating Accelerated Tryptic Digestion into Proteomics Workflows

    • Gordon W. Slysz, David C. Schriemer
    Pages 241-254

  16. Hydrogen/Deuterium Exchange Mass Spectrometry

    • Xuguang Yan, Claudia S. Maier
    Pages 255-271

  17. Mass Spectrometry Detection and Characterization of Noncovalent Protein Complexes

    • Sheng Yin, Joseph A. Loo
    Pages 273-282

  18. Chemical Cross-Linking for Protein–Protein Interaction Studies

    • Xiaoting Tang, James E. Bruce
    Pages 283-293

  19. Tissue Analysis with High-Resolution Imaging Mass Spectrometry

    • A. F. Maarten Altelaar, Ron M. A. Heeren
    Pages 295-308

  20. Proteomic Global Profiling for Cancer Biomarker Discovery

    • Vitor Faca, Hong Wang, Samir Hanash
    Pages 309-320
Back to top

Keywords

About this book

When the last edition of this book was published in 2000, the field of proteomics was in its infancy. At that time, multidimensional liquid chromatographic separations were being introduced as an alternative to traditional gel-based techniques for separating complex protein and peptide mixtures prior to mass spectrometric detection. Today, this approach – referred to as shotgun proteomics – is considered routine for lar- scale global analyses of protein mixtures. Now in its adolescence, proteomics is fundamentally transforming biological and medical research. Much of this transformation can be attributed to technological advancements, particularly in mass spectrometry. Much wider accessibility of hi- resolution and mass measurement accuracy instrumentation in recent years has ini- ated a new revolution in the field by providing more reliable data and shifting the focus from cataloging proteins to precisely quantifying changes in protein abundance over time and in response to stimuli. Advanced mass spectrometers and novel ion d- sociation schemes such as electron transfer/capture dissociation make it possible to venture boldly into the maze of protein posttranslational modifications, which are an integral component of understanding functional proteomics in the spatial and t- poral domains. Another area that has benefited from these advancements is top-down proteomics, an emerging method essential for characterizing various protein variants that has potentially high impact in biomedical research.

Reviews

From the reviews of the second edition:

"This book … covers a wide range of applications of modern mass spectrometry in peptide and protein analysis. … Very useful is the presence of a final ‘Notes’ section within each chapter. These notes and laboratory tips help both beginners and experienced researchers to make their first steps into new fields … . The well balanced mixture … makes it a helpful resource for scientists working in analytical chemistry, biochemistry, biotechnology, and bioinformatics, and a valuable resource for everyone working in biologically oriented mass spectrometry." (Andreas Tholey, Analytical and Bioanalytical Chemistry, September, 2009)

Editors and Affiliations

  • Fundamental & Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, USA

    Mary S. Lipton

  • Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, USA

    Ljiljana Paša-Tolic

Bibliographic Information

  • Book Title: Mass Spectrometry of Proteins and Peptides

  • Book Subtitle: Methods and Protocols, Second Edition

  • Editors: Mary S. Lipton, Ljiljana Paša-Tolic

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-493-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-934115-48-0Published: 18 December 2008

  • Softcover ISBN: 978-1-62703-796-9Published: 27 November 2014

  • eBook ISBN: 978-1-59745-493-3Published: 25 February 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 470

  • Topics: Biochemistry, general, Protein Science, Mass Spectrometry

Publish with us

Policies and ethics

Back to top

Search

Navigation