Abstract
To be able to visualize real time leukocyte — endothelial cell interactions in vitro opens up the possibility of exploring the complex cascade of events that culminate in leukocyte recruitment and diapedesis in a much more detailed and controlled way. Techniques have been developed whereby fluorescently labeled leukocytes are perfused over an endothelial substrate in a controlled manner. Interactions can then be visualized and, using motion tracking software, the movement of cells characterized. Dynamic flow based adhesion assay protocols build on previous static assays of leukocyte adhesion in better modeling the environment in which these interactions actually take place in vivo.
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© 2007 Humana Press Inc., Totowa, NJ
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Florey, O., Haskard, D.O. (2007). Analysis of Flow-Based Adhesion In Vitro. In: Cope, A.P. (eds) Arthritis Research. Methods in Molecular Medicine, vol 135. Humana Press. https://doi.org/10.1007/978-1-59745-401-8_20
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DOI: https://doi.org/10.1007/978-1-59745-401-8_20
Publisher Name: Humana Press
Print ISBN: 978-1-58829-344-2
Online ISBN: 978-1-59745-401-8
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