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Mitochondria pp 529-542 | Cite as

Plant Mitochondrial Transcriptomics by Quantitative RT-PCR

  • Rachel Clifton
  • James Whelan
Part of the Methods in Molecular Biology™ book series (MIMB, volume 372)

Abstract

Transcriptomic analysis using quantitative reverse transcriptase polymerase chain reaction (QRT-PCR) facilitates analysis of nuclear and mitochondrial-encoded mitochondrial genes, enabling mechanisms and regulation of signaling pathways to be explored. To illustrate this technique, we use genes of the mitochondrial respiratory chain. We show that several components of the mitochondrial respiratory chain respond to stress, in particular the alternative oxidase. This chapter describes a method involving total ribonucleic acid (RNA) isolation and QRT-PCR for the detection and analysis of transcriptional changes that accompany seven commonly used chemical stresses. This methodology describes an accurate technique to determine quantitatively absolute transcript levels and a platform to facilitate comparison between responses to other stress stimuli.

Key Words

Alternative respiratory pathway gene expression quantitative RT-PCR 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Rachel Clifton
    • 1
  • James Whelan
    • 2
  1. 1.ARC Centre of Excellence in Plant Energy Biology, M316University of Western AustraliaCrawleyAustralia
  2. 2.Plant Molecular Biology Group, Biochemistry Department, School of Life and Biomedical SciencesUniversity of Western AustraliaCrawleyAustralia

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