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Mitochondria

Practical Protocols

  • Dario Leister
  • Johannes M. Herrmann

Part of the Methods in Molecular Biology™ book series (MIMB, volume 372)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Model Systems to Study Mitochondrial Functions

    1. Front Matter
      Pages 1-1
    2. Florence Malka, Karine Auré, Steffi Goffart, Johannes N. Spelbrink, Manuel Rojo
      Pages 3-16
    3. Steffi Goffart, Peter Martinsson, Florence Malka, Manuel Rojo, Johannes N. Spelbrink
      Pages 17-32
    4. Miguel Angel Fernández-Moreno, Carol L. Farr, Laurie S. Kaguni, Rafael Garesse
      Pages 33-49
    5. Leslie I. Grad, Leanne C. Sayles, Bernard D. Lemire
      Pages 51-66
    6. André Schneider, Fabien Charrière, Mascha Pusnik, Elke K. Horn
      Pages 67-80
    7. Katrin Altmann, Mark Dürr, Benedikt Westermann
      Pages 81-90
    8. Stéphane Chiron, Mauricette Gaisne, Emmanuelle Guillou, Pascale Belenguer, G. Desmond Clark-Walker, Nathalie Bonnefoy
      Pages 91-105
    9. Frank E. Nargang, Doron Rapaport
      Pages 107-123
    10. Lee J. Sweetlove, Nicolas L. Taylor, Christopher J. Leaver
      Pages 125-136
    11. Soledad Funes, Lars-Gunnar Franzén, Diego González-Halphen
      Pages 137-149
  3. Genetics and Molecular Biology

    1. Front Matter
      Pages 151-151
    2. Françoise Foury
      Pages 167-176
    3. Frank Kempken, Nina Bolle, Joachim Forner, Stefan Binder
      Pages 177-192
    4. Elizabeth A. Amiott, Judith A. Jaehning
      Pages 193-206
    5. Yuichi Matsushima, Cristina Adán, Rafael Garesse, Laurie S. Kaguni
      Pages 207-217
    6. Aurelio Reyes, Takehiro Yasukawa, Ian J. Holt
      Pages 219-232
  4. Biochemistry

    1. Front Matter
      Pages 233-233
    2. Anne-Marie Mager-Heckel, Nina Entelis, Irina Brandina, Petr Kamenski, Igor A. Krasheninnikov, Robert P. Martin et al.
      Pages 235-253
    3. Soledad Funes, Johannes M. Herrmann
      Pages 255-263
    4. Dejana Mokranjac, Walter Neupert
      Pages 277-286
    5. Pavel F. Pavlov, Charlotta Rudhe, Shashi Bhushan, Elzbieta Glaser
      Pages 297-314
    6. Oliver Stehling, Paul M. Smith, Annette Biederbick, Janneke Balk, Roland Lill, Ulrich Mühlenhoff
      Pages 325-342
    7. Takashi Tatsuta, Thomas Langer
      Pages 343-360
  5. Physiology

    1. Front Matter
      Pages 361-361
    2. Sharon H. Ackerman, Alexander Tzagoloff
      Pages 363-377
    3. André Schneider, Nabile Bouzaidi-Tiali, Anne-Laure Chanez, Laurence Bulliard
      Pages 379-387
    4. Nicolas L. Taylor, A. Harvey Millar
      Pages 389-403
  6. Dynamics and Morphology

    1. Front Matter
      Pages 431-431
    2. Theresa C. Swayne, Anna C. Gay, Liza A. Pon
      Pages 433-459
    3. Shelly L. Meeusen, Jodi Nunnari
      Pages 461-466
    4. Edward M. Perkins, J. Michael McCaffery
      Pages 467-483
    5. Shilpa Gandre, Alexander M. van der Bliek
      Pages 485-501
  7. Functional Genomics

    1. Front Matter
      Pages 503-503
    2. Mathilde Garcia, Xavier Darzacq, Frederic Devaux, Robert H. Singer, Claude Jacq
      Pages 505-528
    3. Rachel Clifton, James Whelan
      Pages 529-542
    4. Jörg Reinders, Albert Sickmann
      Pages 543-557
    5. Joshua L. Heazlewood, Harvey Millar A.
      Pages 559-571
    6. Holger Prokisch, Uwe Ahting
      Pages 573-586
  8. Back Matter
    Pages 587-592

About this book

Introduction

Mitochondria: Practical Protocols offers a broad collection of methods for studying the molecular biology, function, and features of mitochondria. In the past decade, mitochondrial research has elucidated the important influence of mitochondrial processes on integral cell processes such as apoptosis and cellular aging. This practical guide presents a wide spectrum of mitochondrial methods, each written by specialists with solid experience and intended for implementation by novice and expert researchers alike.

Part I introduces major experimental model systems and discusses their specific advantages and limitations for functional analysis of mitochondria. The concise overview of general properties of mitochondrial systems is supplemented by detailed protocols for cultivation of model organisms. Parts II-VI comprise a robust collection of protocols for studying different molecular aspects of mitochondrial functions including: genetics and microbiology, biochemistry, physiology, dynamics and morphology, and functional genomics. Emphasis is placed on new and emerging topics in mitochondrial study, such as the examination of apoptotic effects, fusion and fission of mitochondria, and proteome and transcriptome analysis.

Keywords

Arabidopsis thaliana DNA PCR Proteomics Vivo biochemistry electron microscopy genetics influence microscopy molecular biology proteins saccharomyces cerevisiae tRNA

Editors and affiliations

  • Dario Leister
    • 1
  • Johannes M. Herrmann
    • 2
  1. 1.Department für Biologie I, BotanikLudwig-Maximilians-Universität MünchenMünchenGermany
  2. 2.ZellbiologieUniversität KaiserslauternKaiserslauternGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-365-3
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-667-2
  • Online ISBN 978-1-59745-365-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site