Abstract
Lentiviruses have the capacity to enter and integrate their genetic material into cells that are not dividing. This property is retained in vectors based on these agents. They can thus effect gene delivery to cells that are difficult to transduce such as cardiac myocytes in vitro and in vivo. They are also relatively efficient at entering dividing cells and can transduce stem cells and vascular endothelium. They have a substantial gene-carrying capacity of up to around 9 kb. They do not trigger an inflammatory response and are thus useful when proinflammatory agents are undesirable, such as in transplantation. Their ease of cloning and well-understood molecular biology have made them highly suitable for gene delivery to the heart.
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References
Zhao, J., Pettigrew, G. J., Thomas, J., et al. (2002) Lentiviral vectors for delivery of genes into neonatal and adult ventricular cardiac myocytes in vitro and in vivo. Basic Res. Cardiol. 97, 348–358.
Dishart, K. L., Denby, L., George, S. J., et al. (2003) Third-generation lentivirus vectors efficiently transduce and phenotypically modify vascular cells: implications for gene therapy. J. Mol. Cell Cardiol. 35, 739–748.
Bonci, D., Cittadini, A., Latronico, M. V., et al. (2003) ‘Advanced’ generation lentiviruses as efficient vectors for cardiomyocyte gene transduction in vitro and in vivo. Gene Ther. 10, 630–636.
Fleury, S., Simeoni, E., Zuppinger, C., et al. (2003) Multiply attenuated, self-inactivating lentiviral vectors efficiently deliver and express genes for extended periods of time in adult rat cardiomyocytes in vivo. Circulation 107, 2375–2382.
Subbramanian, R. A. and Cohen, E. A. (1994) Molecular biology of the human immunodeficiency virus accessory proteins. J. Virol. 68, 6831–6835.
Naldini, L., Blomer, U., Gallay, P., et al. (1996) In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 272, 263–267.
Dull, T., Zufferey, R., Kelly, M., et al. (1998) A third generation lentivirus vector with a conditional packaging system. J. Virol. 72, 8463–8471.
Zufferey, R., Dull, T., Mandel, R. J., et al. (1998) Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery. J. Virol. 72, 9873–9880.
Lever, A., Gottlinger, H., Haseltine, W., and Sodroski, J. (1989) Identification of a sequence required for efficient packaging of human immunodeficiency virus type 1 RNA into virions. J. Virol. 63, 4085–4087.
Kaye, J. F., Richardson, J. H., and Lever, A. M. (1995) Cis-acting sequences involved in human immunodeficiency virus type 1 RNA packaging. J. Virol. 69, 6588–6592.
Berkowitz, R. D., Hammarskjold, M. L., Helga Maria, C., Rekosh, D., and Goff, S. P. (1995) 5′ Regions of HIV-1 RNAs are not sufficient for encapsidation: implications for the HIV-1 packaging signal. Virology 212, 718–723.
Parolin, C., Dorfman, T., Palu, G., Gottlinger, H., and Sodroski, J. (1994) Analysis in human immunodeficiency virus type 1 vectors of cis-acting sequences that affect gene transfer into human lymphocytes. J. Virol. 68, 3888–3895.
D’Agostino, D. M., Felber, B. K., Harrison, J. E., and Pavlakis, G. N. (1992) The Rev protein of human immunodeficiency virus type 1 promotes polysomal association and translation of gag/pol and vpu/env mRNAs. Mol. Cell. Biol. 12, 1375–1386.
Mautino, M. R., Ramsey, W. J., Reiser, J., and Morgan, R. A. (2000) Modified human immunodeficiency virus-based lentiviral vectors display decreased sensitivity to trans-dominant Rev. Hum. Gene Ther. 11, 895–908.
Srinivasakumar, N. and Schuening, F. G. (1999) A lentivirus packaging system based on alternative RNA transport mechanisms to express helper and gene transfer vector RNAs and its use to study the requirement of accessory proteins for particle formation and gene delivery. J. Virol. 73, 9589–9598.
Zufferey, R., Donello, J. E., Trono, D., and Hope, T. J. (1999) Woodchuck hepatitis virus posttranscriptional regulatory element enhances expression of transgenes delivered by retroviral vectors. J. Virol. 73, 2886–2892.
Zennou, V., Petit, C., Guetard, D., Nerhbass, U., Montagnier, L., and Charneau, P. (2000) HIV-1 genome nuclear import is mediated by a central DNA flap. Cell 101, 173–185.
VandenDriessche, T., Thorrez, L., Naldini, L., et al. (2002) Lentiviral vectors containing the human immunodeficiency virus type-1 central polypurine tract can efficiently transduce nondividing hepatocytes and antigen-presenting cells in vivo. Blood 100, 813–822.
Van Maele, B., De Rijck, J., De Clercq, E., and Debyser, Z. (2003) Impact of the central polypurine tract on the kinetics of human immunodeficiency virus type 1 vector transduction. J. Virol. 77, 4685–4694.
Aiken, C. (1997) Pseudotyping human immunodeficiency virus type 1 (HIV-1) by the glycoprotein of vesicular stomatitis virus targets HIV-1 entry to an endocytic pathway and suppresses both the requirement for Nef and the sensitivity to cyclosporin A. J. Virol. 71, 5871–5877.
Yu, H., Rabson, A. B., Kaul, M., Ron, Y., and Dougherty, J. P. (1996) Inducible human immunodeficiency virus type 1 packaging cell lines. J. Virol. 70, 4530–4537.
Carroll, R., Lin, J. T., Dacquel, E. J., Mosca, J. D., Burke, D. S., and St-Louis, D. C. (1994) A human immunodeficiency virus type 1 (HIV-1) based retroviral vector system utilising stable HIV-1 packaging cell lines. J. Virol. 68, 6047–6051.
Ikeda, Y., Takeuchi, Y., Martin, F., Cosset, F. L., Mitrophanous, K., and Collins, M. (2003) Continuous high-titer HIV-1 vector production. Nat. Biotechnol. 21, 569–572.
Haselhorst, D., Kaye, J. F., and Lever, A. M. L. (1998) Development of cell lines stably expressing human immunodeficiency virus type 1 proteins for studies in encapsidation and gene transfer. J. Gen. Virol. 79, 231–237.
Zhao, J., Pettigrew, G. J., Bolton, E. M., et al. (2005) Lentivirus-mediated gene transfer of viral interleukin-10 delays but does not prevent cardiac allograft rejection. Gene Ther. 12, 1509–1516.
Graham, F. L. and van der Eb, A. J. (1973) A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52, 456–467.
Chen, C. and Okayama, H. (1987) High-efficiency transformation of mammalian cells by plasmid DNA. Mol. Cell. Biol. 7, 2745–2752.
Yee, J. K., Friedmann, T., and Burns, J. C. (1994) Generation of high-titer pseudotyped retroviral vectors with very broad host range. Methods Cell Biol. 43 Pt A, 99–112.
Mochizuki, H., Schwartz, J. P., Tanaka, K., Brady, R. O., and Reiser, J. (1998) High-titer human immunodeficiency virus type 1-based vector systems for gene delivery into nondividing cells. J. Virol. 72, 8873–8883.
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Zhao, J., Lever, A.M.L. (2007). Lentivirus-Mediated Gene Expression. In: Zhang, J., Rokosh, G. (eds) Cardiac Gene Expression. Methods in Molecular Biology, vol 366. Humana Press. https://doi.org/10.1007/978-1-59745-030-0_20
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DOI: https://doi.org/10.1007/978-1-59745-030-0_20
Publisher Name: Humana Press
Print ISBN: 978-1-58829-352-7
Online ISBN: 978-1-59745-030-0
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