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Cardiac Gene Expression

Methods and Protocols

  • Jun Zhang
  • Gregg Rokosh

Part of the Methods in Molecular Biology book series (MIMB, volume 366)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Cardiac Gene Expression

    1. Front Matter
      Pages 1-1
    2. Martina Schinke-Braun, Jennifer A. Couget
      Pages 13-40
    3. Kirill V. Tarasov, Sheryl A. Brugh, Yelena S. Tarasova, Kenneth R. Boheler
      Pages 41-59
  3. Cardiac Gene Regulation

    1. Front Matter
      Pages 107-107
    2. Kevin John Ashton, John Patrick Headrick
      Pages 121-143
    3. Yongxia Qu, Mohamed Boutjdir
      Pages 145-158
    4. Thierry P. Calmels, David Mazurais
      Pages 159-180
  4. Cardiac Gene Regulation

    1. Front Matter
      Pages 181-181
    2. Jim Jung-Ching Lin, Shaun E. Grosskurth, Shannon M. Harlan, Elisabeth A. Gustafson-Wagner, Qin Wang
      Pages 183-201
    3. Martin E. Cullen, Paul J. R. Barton
      Pages 203-216
    4. Hsiao-Huei Chen, Alexandre F. R. Stewart
      Pages 217-225
  5. In Silico Assessment of Regulatory cis-Elements and Gene Regulation

    1. Front Matter
      Pages 227-227
    2. Jan-Fang Cheng, James R. Priest, Len A. Pennacchio
      Pages 229-251
    3. Michael B. Bober, Raimond Winslow
      Pages 253-263
  6. Cardiac Single Nucleotide Polymorphisms

    1. Front Matter
      Pages 265-265
    2. Neema Jamshidi, Thuy D. Vo, Bernhard O. Palsson
      Pages 267-285
  7. Gene Overexpression and Targeting in the Myocardium

    1. Front Matter
      Pages 307-307
    2. Kelly R. O’Neal, Ramtin Agah
      Pages 309-320
    3. Alexander H. Maass, Massimo Buvoli
      Pages 321-330
    4. Jing Zhao, Andrew M. L. Lever
      Pages 343-355
  8. Back Matter
    Pages 357-365

About this book

Introduction

Cardiac Gene Expression: Methods and Protocols presents both cutting-edge and established methods for studying cardiac gene expression. The protocols provide a template for solid research, and cover the process through screening, analysis, characterization, and functional confirmation of novel genes or known genes with a new function.

Section I, Cardiac Gene Expression Profiling: The Global Perspective, discusses several different approaches to examining, identifying, and analyzing changes in transcriptome gene expression. Section II, Cardiac Gene Regulation: Gene-Specific mRNA Measurement in the Myocardium, outlines more sensitive and gene-targeted expression methods. Section III, Cardiac Gene Regulation: Promoter Characterization in the Myocardium, provides protocols for the study of underlying gene regulation mechanisms by focusing on the interaction of transcription factors with their cognate cis binding elements. Section IV, In Silico Assessment of Regulatory cis-Elements and Gene Regulation, and Section V, Cardiac Single Network Polymorphisms, emphasize new analytical approaches for deciphering the functional elements buried in the 3 billion nucleotides of the human genome and other model genomes. The concluding section, Gene Overexpression and Targeting in the Myocardium, highlights methods that facilitate overexpression or cardiac-specific targeted gene deletion.

Keywords

DNA In silico Microarray PCR Polymerase Chain Reaction Promoter SNP Single Nucleotide Polymorphism gene expression gene transfer genes genome hybridization regulation mechanisms transcription

Editors and affiliations

  • Jun Zhang
    • 1
  • Gregg Rokosh
    • 2
  1. 1.Division of CardiologyCVRL/Geffen School of Medicine at UCLALos Angeles
  2. 2.Division of CardiologyUniversity of LouisvilleLouisville

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-030-0
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-352-7
  • Online ISBN 978-1-59745-030-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site