How to Study the Proteomes and Phosphoproteomes of Anther and Pollen
Proteomics analysis was a powerful technology for characterizing proteins and protein posttranslational modification (PTMs). Recently, many anther and pollen-related proteomic analyses have been reported, which have expanded our understanding of anther and pollen development and regulation. In this chapter, we describe a detailed, optimized protocol for the separation, digestion, tagging, and subsequent mass spectrometry-based identification and quantification of proteins and phosphoproteins from anther and pollen.
Key wordsAnther Liquid chromatography–mass spectrometry (LC-MS/MS) Pollen Proteome Phosphoproteome
- 15.Zhang Z, Hu M, Feng X, Gong A, Cheng L, Yuan H (2017) Proteomes and phosphoproteomes of anther and pollen: availability and progress. Proteomics 17(20). https://doi.org/10.1002/pmic.201600458