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LC-MS/MS-Based Metabolomics for Cell Cultures

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1994)

Abstract

Metabolomics, a comprehensive analysis of metabolites in biological specimens (e.g., cells, body fluids, tissues, exhaled air, plants), offers promising tools in health, nutrition, biotechnology, and food sciences. Here we describe methods of LC-MS/MS-based analyses for cell metabolomics. Using methods employed in this section, over 1000 endogenous and exogenous metabolites can be detected, annotated, and quantified relatively by nontargeted analysis approach, whereas targeted metabolomics analysis enables us to quantify 188 endogenous metabolites.

Key words

Metabolomics Mass spectrometry LC-MS/MS-based metabolomics Cell culture metabolomics Nontargeted metabolomics Targeted metabolomics 
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Notes

Acknowledgments

The research leading to these results has received support from the Innovative Medicines Initiative Joint Undertaking under grant agreement no. 115439, resources of which are composed of financial contribution from the European Union’s Seventh Framework Programme (FP7/2007-2013) and EFPIA companies’ in-kind contribution.

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Research Unit Molecular Endocrinology and MetabolismHelmholtz Zentrum München, German Research Center for Environmental Health (GmbH)NeuherbergGermany
  2. 2.German Center for Diabetes Research (DZD e.V.)NeuherbergGermany
  3. 3.Lehrstuhl für Experimentelle GenetikTechnische Universität MünchenFreising-WeihenstephanGermany
  4. 4.Department of BiochemistryYong Loo Lin School of MedicineNational University of SingaporeSingapore

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