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Analysis of Redox Relationships in the Plant Cell Cycle: Determination of Ascorbate, Glutathione, and Poly(ADPribose)polymerase (PARP) in Plant Cell Cultures

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Redox-Mediated Signal Transduction

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1990))

Abstract

Reactive oxygen species (ROS) and low molecular weight antioxidants, such as glutathione and ascorbate, are powerful signalling molecules that participate in the control of plant growth and development, and modulate progression through the mitotic cell cycle. Enhanced ROS accumulation or low levels of ascorbate or glutathione cause the cell cycle to arrest and halt progression especially through the G1 checkpoint. Plant cell suspension cultures have proved to be particularly useful tools for the study of cell cycle regulation. Here we provide effective and accurate methods for the measurement of changes in the cellular ascorbate and glutathione pools and the activities of related enzymes such poly(ADP-ribose)polymerase (PARP) during mitosis and cell expansion, particularly in cell suspension cultures. These methods can be used in studies seeking to improve current understanding of the roles of redox controls on cell division and cell expansion.

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Author information

Authors and Affiliations

  1. Faculty of Biological Sciences, University of Leeds, Leeds, UK

    Christine H. Foyer, Till K. Pellny, Vittoria Locato, Jonathon Hull & Laura De Gara

  2. Faculty Health and Applied Sciences, University of the West of England, Bristol, UK

    Jonathon Hull

Authors
  1. Christine H. Foyer
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  2. Till K. Pellny
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  3. Vittoria Locato
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  4. Jonathon Hull
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  5. Laura De Gara
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Corresponding author

Correspondence to Christine H. Foyer .

Editor information

Editors and Affiliations

  1. Department of Applied Sciences, University of the West of England, Bristol, UK

    John T. Hancock

  2. Department of Applied Sciences, University of the West of England, Bristol, UK

    Myra E. Conway

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Foyer, C.H., Pellny, T.K., Locato, V., Hull, J., De Gara, L. (2019). Analysis of Redox Relationships in the Plant Cell Cycle: Determination of Ascorbate, Glutathione, and Poly(ADPribose)polymerase (PARP) in Plant Cell Cultures. In: Hancock, J., Conway, M. (eds) Redox-Mediated Signal Transduction. Methods in Molecular Biology, vol 1990. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9463-2_14

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  • DOI: https://doi.org/10.1007/978-1-4939-9463-2_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9461-8

  • Online ISBN: 978-1-4939-9463-2

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