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Reconstitution of Intracellular Calcium Signaling in Xenopus Egg Extracts

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Vertebrate Embryogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1920))

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Abstract

Generation of calcium signal in the cytoplasm of fertilized or parthenogenetically activated eggs has been extensively studied in the intact eggs of several biological species. Calcium transient was found to elicit a plethora of biochemical and cellular events in these cells. Remarkably, intracellular calcium signaling can also be reconstituted in cell-free environment. In this chapter, we describe the methods that allow reconstitution, detection, and quantification of the calcium signal in cell-free extracts of Xenopus oocytes and eggs.

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Acknowledgments

This work was supported by the Collaboration Research grant 281027 from Kobe University to A.A.T. and the Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan 15K07083 to K-I.S.

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Authors and Affiliations

  1. Laboratory of Cell Signaling and Development, Department of Molecular Biosciences, Faculty of Life Sciences, Kyoto Sangyo University, Kyoto, Japan

    Alexander A. Tokmakov & Ken-Ichi Sato

Authors
  1. Alexander A. Tokmakov
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  2. Ken-Ichi Sato
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Corresponding author

Correspondence to Alexander A. Tokmakov .

Editor information

Editors and Affiliations

  1. Laboratory of Genetics, University of Wisconsin–Madison, Madison, WI, USA

    Francisco J. Pelegri

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Tokmakov, A.A., Sato, KI. (2019). Reconstitution of Intracellular Calcium Signaling in Xenopus Egg Extracts. In: Pelegri, F. (eds) Vertebrate Embryogenesis. Methods in Molecular Biology, vol 1920. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9009-2_4

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  • DOI: https://doi.org/10.1007/978-1-4939-9009-2_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9008-5

  • Online ISBN: 978-1-4939-9009-2

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