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The Use of Multiplex Real-Time PCR for the Simultaneous Detection of Foodborne Bacterial Pathogens

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Foodborne Bacterial Pathogens

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1918))

Abstract

Foodborne pathogens continue to be a major health issue worldwide. Culture-dependent methodologies are still considered the gold-standard to perform pathogen detection and quantification. These methods present several drawbacks, such as being time-consuming and labor-intensive. The implementation of real-time PCR has allowed to overcome these limitations and even reduce costs associated with the analyses, due to the possibility of simultaneously and accurately detecting several pathogens in one single assay, with results comparable to those obtained by classical approaches. In this chapter a protocol for the simultaneous detection of two of the most important foodborne pathogens, Salmonella spp. and Listeria monocytogenes, is described.

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Author information

Authors and Affiliations

  1. Department of Life Sciences, Food Quality and Safety Research Group, International Iberian Nanotechnology Laboratory, Braga, Portugal

    Alejandro Garrido-Maestu & Marta Prado Rodríguez

  2. Nestlé Research Center, Institute Food Safety and Analytical Science, Microbial and Molecular Analytics Group, Lausanne, Switzerland

    David Tomás Fornés

Authors
  1. Alejandro Garrido-Maestu
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  2. David Tomás Fornés
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  3. Marta Prado Rodríguez
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Corresponding author

Correspondence to Alejandro Garrido-Maestu .

Editor information

Editors and Affiliations

  1. Fougères Laboratory, ANSES, Fougères, France

    Arnaud Bridier

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© 2019 Springer Science+Business Media, LLC, part of Springer Nature

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Garrido-Maestu, A., Tomás Fornés, D., Prado Rodríguez, M. (2019). The Use of Multiplex Real-Time PCR for the Simultaneous Detection of Foodborne Bacterial Pathogens. In: Bridier, A. (eds) Foodborne Bacterial Pathogens. Methods in Molecular Biology, vol 1918. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9000-9_3

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  • DOI: https://doi.org/10.1007/978-1-4939-9000-9_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-8999-7

  • Online ISBN: 978-1-4939-9000-9

  • eBook Packages: Springer Protocols

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