Abstract
The X. laevis sub-capsular liver is thought to be the principal hematopoietic site of Xenopodinae species from early development and, in case of certain species, into adulthood. The Xenopus bone marrow appears to be comprised of precursor cells committed to myeloid lineages, such as macrophage- and granulocyte-progenitor cells. With alarming increases in the contribution of pathogenic infections to the global amphibian declines, now more than ever a better understanding of the mechanisms controlling amphibian immune cell ontogeny and functionality is warranted. Accordingly, here we detail the isolation and culture of the X. laevis hematopoietic cells from the sub-capsular liver and bone marrow. Considering the immunological roles attributed to these amphibian organs, the respective cell isolation protocols described here will be pertinent to garnering further insights into the coordinated regulation of amphibian hematopoiesis and immune defense mechanisms.
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References
Akulenko N (2012) Haemopoietic system of the anurans: the role of bone marrow and liver. Vestn Zool 46:28–35
Carver FJ, Meints RH (1977) Studies of the development of frog hemopoietic tissue in vitro. I. Spleen culture assay of an erythropoietic factor in anemic frog blood. J Exp Zool 201:37–46
Golub R, André S, Hassanin A et al (2004) Early expression of two TdT isoforms in the hematopoietic system of the Mexican axolotl. Implications for the evolutionary origin of the N-nucleotide addition. Immunogenetics 56:204–213
Lane MC, Sheets MD (2002) Primitive and definitive blood share a common origin in Xenopus: a comparison of lineage techniques used to construct fate maps. Dev Biol 248:52–67
Nogawa-Kosaka N, Sugai T, Nagasawa K et al (2011) Identification of erythroid progenitors induced by erythropoietic activity in Xenopus laevis. J Exp Biol 214:921–927
Chen XD, Turpen JB (1995) Intraembryonic origin of hepatic hematopoiesis in Xenopus laevis. J Immunol 154:2557–6257
Lopez D, Lin L, Monaghan JR et al (2014) Mapping hematopoiesis in a fully regenerative vertebrate: the axolotl. Blood 124:1232–1241
de Abreu Manso PP, de Brito-Gitirana L, Pelajo-Machado M (2009) Localization of hematopoietic cells in the bullfrog (Lithobates catesbeianus). Cell Tissue Res 337:301–312
Tanaka Y (1976) Architecture of the marrow vasculature in three amphibian species and its significance in hematopoietic development. Am J Anat 145:485–497
Manning MJ, Horton JD (1969) Histogenesis of lymphoid organs in larvae of the south African clawed toad, Xenopus laevis (Daudin). J Embryol Exp Morphol 22:265–277
Acknowledgment
AY thanks the GWU Department of Biological Sciences of Teaching and Research Stipends and thanks the Harlan Research Fund for summer research support. LG thanks the GWU Department of Biological Sciences for research support at the new investigator level. AY and LG thank Yaprak Ozakman for her assistance with generating the figures presented here.
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Yaparla, A., Grayfer, L. (2018). Isolation and Culture of Amphibian (Xenopus laevis) Sub-Capsular Liver and Bone Marrow Cells. In: Vleminckx, K. (eds) Xenopus. Methods in Molecular Biology, vol 1865. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8784-9_20
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DOI: https://doi.org/10.1007/978-1-4939-8784-9_20
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-8784-9
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